货号：700360

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 UG

目录价：￥4692.00

市场价格：￥4222.80

会员价格：￥4222.80

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 ABfinity™ Recombinant Rabbit Monoclonal Antibody is validated with human and expected to detect Rhesus monkey, mouse, rat, swine, bovine MDA5. This antibody is validated for use in Western Blot and Immunofluorescence. MDA5 is encoded by the 64135 gene, also known as Hlcd, MDA5, MDA-5, IDDM19, MGC133047, IFIH1.Melanoma differentiation associated gene 5, MDA5, is a pattern recognition receptor (PRR) involved in the recognition of viral dsRNA (1). Along with RIG-I, MDA5 detects viral dsRNA and activates the innate immune response. Both MDA5 and RIG-I are RNA helicases and they perform overlapping as well as distinct roles. RIG-I is activated by dsRNAs without a 5’-triphosphate end and short dsRNAs, whereas MDA5 is activated by long dsRNAs (2). Like RIG-I, MDA5 uses a DExD⁄H-Box helicase motif to sense viral RNA and an N-terminal caspase recruitment domain (CARD) to transmit the signal (1).Once activated, both proteins signal through IPS-1 activating transcription factors NF-κB and IRF-3 (3) and ultimately activating apoptosis, cytokine signaling, and inflammation (4). RIG-I is essential for signaling by influenza A, influenza B, human respiratory syncytial virus (4), paromyxoviruses, Japanese encephalitis virus (5). MDA5 is essential for signaling by picornavirus (5). Both RIG-I and MDA5 are responsible for signaling by West Nile virus (6).Yoneyama, M. et al. (2005) Shared and unique functions of the DExD⁄H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity. J. Immunol. 2851-2858.Kato, H. et al. (2008) Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid-inducible gene-I and melanoma differentiation-associated gene 5. J. Exp. Med. 205: 1601-1610.Yoneyama, M et al (2004) The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol. 5: 730-736.Loo, Y-M. et al. (2008) Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity. J. Virol. 82: 335-345.Kato, H. et al. (2006) Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. Nature 441: 101-105.Fredericksen, B.L. (2008) Establishment and maintenance of the innate antiviral response to West Nile virus involves both RIG-I and MDA5 signaling through IPS-1. J. Virol. 82:609-616. 规格

Reactivity:	Human
Validated Application:	Western Blot,Immunofluorescence
Host:	Rabbit
SubClass (Isotype):	IgG
Clonality:	Monoclonal
Clone ID:	33H12L34
Antibody Fragment:	Whole Antibody
Target Gene:	MDA5
Gene ID:	64135
Accession Number:	Q9BYX4
Label or Dye:	Unconjugated
Purification:	Affinity Purified
Concentration:	0.5 mg⁄ml
Form:	Liquid
Neutralizing Antibody:	No
Product Size:	100 µg
Quantity:	1 each
Volume:	200 µl
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 2-8°C for up to 1 month, -20°C for long term storage. Avoid repeated freezing and thawing.