货号：700582

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 UG

目录价：￥4692.00

市场价格：￥4222.80

会员价格：￥4222.80

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 This purified ABfinity™ recombinant rabbit monoclonal antibody recognizes PRKC1 [pT555/pT563] in human and mouse.ABfinity™ PKCiota[pT555]/PKClambda[pT563] antibody characteristics include:• Excellent lot-to-lot consistency• Extremely high specificity and sensitivity• Recombinant rabbit IgG• Validation in Western blotPhospho-Specific Anti- PKCiota[pT555]/PKClambda[pT563]PRKC1 belongs to the protein kinase C family of serine/threonine protein kinases which play a pivotal role in various cellular functions including cell growth and differentiation, gene expression, and hormone secretion (1-2). Recently PRKC1 has been described as an oncogene and has been connected to cancers as a prognostic marker and therapeutic target (3). PRKC1 has been linked to heat shock response and implicated as an indirect co-regulator of HSF1 activity, thus PRKC1 presents as a new potential therapeutic target and focus of gene-environment interactions (4). PKCiota[pT555]/PKClambda[pT563] is encoded by the 5584 gene, also known as protein kinase C iota. Protein kinase C iota/lambda phosphorylates glyceraldehyde-3-phosphate dehydrogenase thus playing an important role in microtubule dynamics during the early secretory pathway (5).Exceptional Consistency Saves You Time and Money ABfinity™ antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.High Specificity Means More Reliable DataThe proprietary recombinant technology employed in the production of ABfinity™ antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Invitrogen has validated this antibody in Western blotting (Figure 1). High Sensitivity Lets You Detect Low Levels of Target ProteinABfinity™ antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.Recombinant Rabbit IgG--Use Like Any Other IgG AntibodyAs with traditional IgG antibodies, gel electrophoresis of ABfinity™antibodies produces a 150kDa band under non-reducing conditions and 50kDa and 25kDa bands under reducing conditions.This product is for research use only. Not for use in diagnostic proceduresLearn more about the superior performance of ABfinity™ recombinant monoclonal antibodiesSee Invitrogen’s full line of ABfinity™ recombinant monoclonal antibodiesReferences1) Mellor, H., and Parker, P.J. (1998). The extended protein kinase C superfamily. The Biochemical journal 332 ( Pt 2), 281-2922) Nishizuka, Y. (1984). The role of protein kinase C in cell surface signal transduction and tumor promotion. Nature 308, 693-698.3) Fields, A.P., and Regala, R.P. (2007). Protein kinase C iota: human oncogene, prognostic marker and therapeutic target. Pharmacol Res 55, 487-4974) Boellmann, F., and Thomas, R.S. The identification of protein kinase C iota as a regulator of the Mammalian heat shock response using functional genomic screens. PloS one 5, e11850.5) Tisdale, E.J. (2002). Glyceraldehyde-3-phosphate dehydrogenase is phosphorylated by protein kinase C iota /lambda and plays a role in microtubule dynamics in the early secretary pathway. The Journal of biological chemistry 277, 3334-3341 规格

Reactivity:	Human
Validated Application:	Western Blot
Host:	Rabbit
SubClass (Isotype):	IgG
Clonality:	Monoclonal
Clone ID:	31H34L29
Antibody Fragment:	Whole Antibody
Target Gene:	PKC Iota [pT555]/PKC Lambda [pT563]
Gene ID:	5584
Gene Symbol:	PRKCI
Accession Number:	P41743
Label or Dye:	Unconjugated
Target Modification State:	Phospho
Purification:	Purified
Concentration:	0.5 mg⁄ml
Form:	Frozen
Mass:	100 µg
Neutralizing Antibody:	No
Product Size:	100 μg
Protein Family:	Kinases & Inhibitors
Volume:	200 µl
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 2-8°C for up to 1 month, -20°C for long term storage. Avoid repeated freezing and thawing.