货号：700662

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 UG

目录价：￥4692.00

市场价格：￥4222.80

会员价格：￥4222.80

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 This purified ABfinity™ recombinant rabbit monoclonal antibody recognizes the IRS-1 protein in human samples.ABfinity™ antibody characteristics include:• Validation in Western blot• Superior lot-to-lot consistency• Extremely high specificity and sensitivity• Recombinant rabbit monoclonal antibodySpecific for IRS1Insulin Receptor Substrate-1 (IRS-1) is a 180 kDa cytoplasmic protein involved in metabolic and proliferative signaling by insulin, IL-4, and other cytokines (Ref 1,2). The main function of IRS-1 is to enhance growth hormone-induced proliferative signaling (Ref 3,4). The activated insulin receptor phosphorylates IRS proteins on multiple tyrosine residues that serve as docking sites for downstream mediators of metabolic actions such as phosphatidylinositol-3 kinase (Ref 4,5). IRS proteins also undergo serine phosphorylation, which regulates their functions (Ref 6). Phosphorylation of human IRS-1 at serine residues, such as 312 and 616 (serines 307 and 612 in mouse), results in the impairment of metabolic insulin signaling pathways (Ref 7).Exceptional Consistency Saves You Time and MoneyABfinity™ antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.High Specificity Means More Reliable DataThe proprietary recombinant technology employed in the production of ABfinity™ antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Invitrogen has validated this antibody in Western blotting.High Sensitivity Lets You Detect Low Levels of Target ProteinABfinity™ antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.Recombinant Rabbit IgG—Use Like Any Other IgG AntibodyAs with traditional IgG antibodies, gel electrophoresis of ABfinity™ antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.For research use only. Not intended for any human or animal therapeutic or diagnostic use.Related Links:Learn more about the superior performance of ABfinity™ Recombinant Monoclonal AntibodiesSee Invitrogen’s full line of ABfinity™ Recombinant Monoclonal AntibodiesReferences:1. Zick Y. Uncoupling insulin signaling by serine/threonine phosphorylation: a molecular basis for insulin resistance. Biochem Soc Trans. 2004 Nov;32 (Pt 5):812-6. Review. PubMed PMID: 15494022.2. Hers I, Bell CJ, Poole AW, Jiang D, Denton RM, Schaefer E, Tavaré JM. Reciprocal feedback regulation of insulin receptor and insulin receptor substrate tyrosine phosphorylation by phosphoinositide 3-kinase in primary adipocytes. Biochem J. 2002 Dec 15;368(Pt 3):875-84. PubMed PMID: 12220227.3. Fujioka T, Kim JH, Adachi H, Saito K, Tsujimoto M, Yokoyama S, Ui M. Further evidence for the involvement of insulin receptor substrates in epidermal growth factor-induced activation of phosphatidylinositol 3-kinase. Eur J Biochem. 2001 Aug;268(15):4158-68. PubMed PMID: 11488908.4. Aguirre V, Werner ED, Giraud J, Lee YH, Shoelson SE, White MF. Phosphorylation of Ser307 in insulin receptor substrate-1 blocks interactions with the insulin receptor and inhibits insulin action. J Biol Chem. 2002 Jan 11;277(2):1531-7. Epub 2001 Oct 17. PubMed PMID: 11606564.5. Reiss K, Wang JY, Romano G, Tu X, Peruzzi F, Baserga R. Mechanisms of regulation of cell adhesion and motility by insulin receptor substrate-1 in prostate cancer cells. Oncogene. 2001 Jan 25;20(4):490-500. PubMed PMID: 11313980.6. 1: Ravichandran LV, Esposito DL, Chen J, Quon MJ. Protein kinase C-zeta phosphorylates insulin receptor substrate-1 and impairs its ability to activate phosphatidylinositol 3-kinase in response to insulin. J Biol Chem. 2001 Feb 2;276(5):3543-9. Epub 2000 Nov 3. PubMed PMID: 11063744.7. 1: Li J, DeFea K, Roth RA. Modulation of insulin receptor substrate-1 tyrosine phosphorylation by an Akt/phosphatidylinositol 3-kinase pathway. J Biol Chem. 1999 Apr 2;274(14):9351-6. PubMed PMID: 10092613. 规格

Reactivity:	Human
Validated Application:	Western Blot
Host:	Rabbit
SubClass (Isotype):	IgG
Clonality:	Monoclonal
Clone ID:	21H10L24
Antibody Fragment:	Whole Antibody
Gene ID:	3667
Gene Symbol:	IRS1
Accession Number:	P35568
Label or Dye:	Unconjugated
Purification:	Purified
Concentration:	0.5 mg⁄ml
Color:	Unconjugated
Mass:	100 µg
Neutralizing Antibody:	No
Product Size:	100 μg
Volume:	200 µl
Research Category:	Signal Transduction
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 2-8°C for up to 1 month, -20°C for long term storage. Avoid repeated freezing and thawing.