货号：700712

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 UG

目录价：￥4772.00

市场价格：￥4294.80

会员价格：￥4294.80

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 This purified ABfinity™ recombinant rabbit monoclonal antibody recognizes LC3B.ABfinity™ antibody characteristics include:• Validation in Western blot• Superior lot-to-lot consistency• Extremely high specificity and sensitivity• Recombinant rabbit monoclonal antibodySpecific for LC3BLC3B (Autophagy Marker Light Chain 3B), in humans, is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B) (Ref 1,2). LC3B is associated with microtubule assembly and is important in neurogenesis. Recent studies indicates that LC3B plays a vital role in autophagy, a process that involves the bulk degradation of cytoplasmic component (Ref 3,4). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (Ref 5).Exceptional Consistency Saves You Time and MoneyABfinity™ antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.High Specificity Means More Reliable DataThe proprietary recombinant technology employed in the production of ABfinity™ antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Invitrogen has validated this antibody in Western blotting (Fig 1).High Sensitivity Lets You Detect Low Levels of Target ProteinABfinity™ antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.Recombinant Rabbit IgG--Use Like Any Other IgG AntibodyAs with traditional IgG antibodies, gel electrophoresis of ABfinity™ antibodies produces a 150kDa band under non-reducing conditions and 50kDa and 25kDa bands under reducing conditions.For research use only. Not intended for human or animal therapeutic or diagnostic use.Related Links:Learn more about the superior performance of ABfinity™ recombinant monoclonal antibodiesSee Invitrogen's full line of ABfinity™ recombinant monoclonal antibodiesReferences:1. Mann SS, Hammarback JA. Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B. J Biol Chem. 1994 Apr 15;269(15):11492-7. PubMed PMID: 79089092. Tanida I, Ueno T, Kominami E. Human light chain 3/MAP1LC3B is cleaved at its carboxyl- terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes. J Biol Chem. 2004 Nov 12;279(46):47704-10. Epub 2004 Sep 7. PubMed PMID: 153559583. Behrends C, Sowa ME, Gygi SP, Harper JW. Network organization of the human autophagy system. Nature. 2010 Jul 1;466(7302):68-76. Epub 2010 Jun 20. PubMed PMID: 20562859.4. Tanida I, Ueno T, Kominami E. LC3 conjugation system in mammalian autophagy. Int J Biochem Cell Biol. 2004 Dec;36(12):2503-18. Review. PubMed PMID: 153255885. He H, Dang Y, Dai F, Guo Z, Wu J, She X, Pei Y, Chen Y, Ling W, Wu C, Zhao S, Liu JO, Yu L. Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B. J Biol Chem. 2003 Aug 1;278(31):29278-87. Epub 2003 May 11. PubMed PMID: 12740394 规格

Reactivity:	Human
Validated Application:	Western Blot
Host:	Rabbit
SubClass (Isotype):	IgG
Clonality:	Monoclonal
Clone ID:	2H30L32
Antibody Fragment:	Whole Antibody
Gene ID:	81631
Gene Symbol:	MAP1LC3B
Accession Number:	Q9GZQ8
Label or Dye:	Unconjugated
Purification:	Affinity Purified
Concentration:	0.5 mg⁄ml
Form:	Liquid
Mass:	100 µg
Neutralizing Antibody:	No
Product Size:	100 µg
Quantity:	1 vial(s)
Volume:	200 µl
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 2-8°C for up to 1 month, -20°C for long term storage. Avoid repeated freezing and thawing.