货号：340512

品牌：BD IS

规格：50T

目录价：￥3024.00

市场价格：￥2116.80

会员价格：￥1965.60

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Catalog Number

340512

Brand

BD FastImmune™

Name

TNF-α

Size

50Tests

RegulatoryStatus

RUO (GMP)

Clone

6401.1111

Format

PE

Isotype

IgG₁, κ

W.S. No

none

W.B. Positive Control

none

Molecular Weight

none

Reactivity

QC Testing : Human

Applications

Flow cytometry	- Routinely Tested
Intracellular Staining	- Routinely Tested

Clone - Description

Anti-Human Tumor Necrosis Factor-a (Anti-Hu–TNF-a) recognizes a 26-kDa transmembrane protein.Tumor Necrosis Factor-a (TNF-a), formerly known as cachectin, is a pleiotropic cytokine that is a major mediator of inflammatory, immunological, and pathophysiological reactions. Two distinct species of the molecule exist, a 26-kDa membrane expressed form (mTNF) and a 17-kDa soluble form (sTNF). The sTNF is derived from the proteolytic cleavage of the mTNF. Both forms are bioactive, with the active form of the sTNF being composed of a trimer of identical 17-kDa subunits. The 17-kDa form, bound to its receptor, can also be detected on the membrane of activated cells. There are two distinct membrane receptors for TNF, both of which help to mediate the cellular effects of TNF-a. One receptor has a molecular weight of 55 to 60 kDa (p55 or TNF-R1) and the other receptor has a molecular weight of 70 to 80 kDa (p75 or TNF-R2).TNF-a is primarily produced by activated lymphocytes and macrophages. In T lymphocytes, TNF-a can be induced through different molecular pathways, including the AIM/CD69 activation pathway and the CD3 and CD28 pathway. Lipopolysaccharide (LPS) and other endotoxins are potent triggers of TNF-a from macrophages/monocytes.TNF-a is a potent proinflammatory polypeptide that exhibits multiple biological activities. It contributes to the defense against infectious agents and the control of tumor growth. TNF-a displays antiviral activity, has a role in enhancing eosinophil function, and is involved in the activation of antileishmanial defense. Conversely, TNF-a is implicated as a major mediator of septic shock, rheumatoid arthritis, inflammatory tissue destruction, and of cachexia associated with chronic disease states. It has been postulated that different forms of the TNF molecule induce different physiologic responses, with the cell-bound version (26 kDa) being advantageous to the host and the secreted version (17 kDa) being deleterious.