货号：MAB1012

品牌：Merck Millipore

规格：100UL

目录价：￥2903.00

市场价格：￥2467.55

会员价格：￥2322.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Alkaline Phosphatase Antibody, E. coli, bacterial only | MAB1012 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Alkaline phosphatase (AP). MAB1012 has a high affinity and recognizes an AP determinant resistant to denaturation by SDS-PAGE. It is therefore ideally suited for sensitive and specific detection of AP fusion proteins by Western blot analysis of E. coli transformants expressing fusion products. View All» Epitope: E. coli, bacterial only View All» Immunogen: F201C1B View All» Isotype: IgG2a View All» Species Reactivity: E. coli View All» Application Notes: Western blot at 1:5,000. 42-45kDa on SDS-PAGE,reducing gels for natural E. coli alkaline phosphatase monomer. Alkaline phosphatase fusion proteins will vary depending upon the target fusion protein. Immunocytochemistry: reacts with E.coli. AP fusion protein targets in acetone fixed cell preparations. 1:4000, other fixatives or conditions untested. ASSAY: Preparation of E. coli TnphoA transformants: E. coli strain CC118 was transformed with plasmid pGEM-3Z containing TnphoA insertional mutations in the p101 gene of Mycoplasma hyorhinis, which encodes a protein with a typical N-terminal prokaryotic single peptide (Yogev et al. 1991). Identification of fusion protein with MAB1012 transformants: Transformants are grown in 2XYT medium to OD600=0.6. Cells were centrifuged 3 minutes at 10,000 x g, suspended in SDS-PAGE sample buffer, heated at 100°C for 5 minutes, frozen and thawed and centrifuged as above at room temperature to remove insoluble material. The sample is applied at 9% to a SDS-PAGE gel, and Western immunoblot is performed as described (Yogev et al. 1991). Immunoprecipitation: 5μL of antibody per 500μL of lysate in RIPA or 0.5% triton X-100 solutions. Optimal working dilutions must be determined by end user. View All» Presentation: Ascites. Contains no preservative. View All» Storage Conditions: Store at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P00634 View All» Entrez Gene Number: 945041 View All» Alternate Names: AP; Alk. Phos. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Immunoprecipitation

• Western Blotting

• Immunocytochemistry
  View All» Entrez Gene Summary: Alkaline phosphatases are non-specific phosphomonoesterases that catalyze the hydrolysis reaction via a phosphoseryl intermediate to produce inorganic phosphate and the corresponding alcohol, optimally at high pH. Alkaline phosphatase exists as a dimer, each monomer binding 2 zinc atoms and one magnesium atom, which are essential for enzymatic activity. View All» UniProt Summary: FUNCTION: SwissProt: P00634# alkaline phosphatase activityCOFACTOR: Binds 1 magnesium ion; Binds 2 zinc ions.SIZE: 471 amino acids; 49,439 Da SUBUNIT: Homodimer.SUBCELLULAR LOCATION: Periplasm, Periplasmic space.PTM: Disulfide bond; Phosphoprotein.SIMILARITY: SwissProt: P00634 ## Belongs to the alkaline phosphatase family. View All» Brand Family: Chemicon View All» Product Name: Anti-Alkaline Phosphatase Antibody, E. coli, bacterial only | MAB1012 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Ascites View All» Host: Mouse View All»
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