货号：MAB1037-250UG

品牌：Merck Millipore

规格：

目录价：￥3346.00

市场价格：￥2844.10

会员价格：￥2676.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-GM-CSF-α Receptor Antibody, clone K12B7.17A | MAB1037-250UG View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: MAB1037 neutralizes the human native granulocyte-macrophage colony stimulating factor (GM-CSF) receptor and recombinant GM-CSF receptor. Binds to the alpha chain of human GM-CSF receptor inhibiting both high-affinity (alpha-beta chain heterodimer) and low-affinity (alpha chain only) binding and subsequent action of GM-CSF(Nicola, 1993). This antibody has a relatively high-affinity for the GM-CSF receptor alpha chain (KD-3nM). View All» Clone: K12B7.17A View All» Isotype: IgG2a View All» Species Reactivity: Human View All» Application Notes: Applications include neutralizing the activity of GM CSF and inhibiting human GM-CSF biological action, regardless of whether low-affinity (a-chain only) or high-affinity (a-beta chain heterodimer) receptors are present. The antibody can also be used for Western blot analysis, immunoprecipitation and FACS-flow cytometry. PROTOCOLS: FACS Analysis 1. Incubate 106 cells with 10 μg/mL of antibody at 4°C for 30 min, then wash. 2. Incubate with fluorescein isothiocyanate (FITC) labelled F(ab)2 fragment of sheep ant-mouse Ig antibody at 4°C for 30 min, then wash. Immunoprecipitation 1. Lyse surface iodinated cells (107- 108) in 1% Triton X-100. 2. Incubate the pre-cleared lysate with the antibody at 10 μg/mL for 1 hr at 4°C. 3. Incubate the antibody complexes with Protein-A-Sepharose™ for 1 hr at 4°C. 4. Recover the beads by centrifugation, wash, and then elute with SDS sample buffer prior to running SDS-PAGE. Neutralization The anti GM-CSF receptor (neutralizing) antibody is a potent inhibitor of GM-CSF biological action in several different bioassays with a half maximal inhibitory dose of 1 μg/mL (6 nM) including: 1. Human GM-CSF stimulated 7-day and 14-day bone marrow colonies cultured in agar. 2. Tritiated thymidine incorporation by AML 193 cells. 3. Colony formation by human primary bone marrow cells, and colony formation by FDC-PI cells transfected with the human GM-CSF human receptor a-chain. 4. 125I-human-GM-CSF binding to low-affinity or high-affinity receptors. Western Blot 1. Electroblot SDS-PAGE gels to nitrocellulose sheet and block with 5% w/v milk powder. 2. Incubate nitrocellulose sheet with antibody at 10 μg/mL at room temperature for 1-2 hrs. 3. Incubate nitrocellulose sheet with horseradish peroxidase-labelled rabbit anti-mouse Ig for approximately 1 hr at room temperature. 4. Wash and then develop using Enhanced Chemiluminescence for 5-10 min. View All» Presentation: MAB1037 is 0.22 micron filtered in phosphate buffered saline (PBS) with endotoxin levels 95% pure as determined by View All» Storage Conditions: Anti-GM-CSF receptor (neutralizing) is shipped liquid at ambient temperature. Store at 2-8°C for up to 6 months. View All» UniProt Number: P15509 View All» Entrez Gene Number:

• NM_172248.1

• NM_006140.3

• NM_172245.1

• NM_172246.1

• NM_172247.1

• NM_172249.1
  View All» Gene Symbol:
  • CSF2RA

  • CSF2R

  • CSF2RAY

  • GMR

  • CSF2RX

  • MGC4838

  • GM-CSF-R-alpha

  • CSF2RAX

  • CD116

  • MGC3848

  • CSF2RY

  • CDw116

  • GMCSFR
    View All» Alternate Names: CD116 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Flow Cytometry

    • Immunoprecipitation

    • Western Blotting

    • Neutralizing
      View All» Entrez Gene Summary: The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble. View All» UniProt Summary: FUNCTION: SwissProt: P15509 # Low affinity receptor for granulocyte-macrophage colony- stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.SIZE: 400 amino acids; 46207 Da SUBUNIT: Heterodimer of an alpha and a beta chain. The beta chain is common to the IL3, IL5 and GM-CSF receptors.SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 3: Secreted (Probable). & Isoform 4: Secreted (Probable). & Isoform 6: Secreted (Probable).DOMAIN: SwissProt: P15509 The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell- surface receptor binding. & The box 1 motif is required for JAK interaction and/or activation.SIMILARITY: Belongs to the type I cytokine receptor family. Type 5 subfamily. MISCELLANEOUS: The gene encoding for this protein is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. View All» Brand Family: Chemicon View All» Product Name: Anti-GM-CSF-α Receptor Antibody, clone K12B7.17A | MAB1037-250UG View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 250 µg View All» Format: Purified View All» Host: Mouse View All»
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