货号：MAB1044

品牌：Merck Millipore

规格：250Ug

目录价：￥4771.00

市场价格：￥4055.35

会员价格：￥3816.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Interleukin-8 Antibody, clone 8M6 | MAB1044 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reacts with ovine IL-8. No reactivity detected to recombinant ovine IL-1beta, IL-6, MCP-1 or TNFalpha. View All» Immunogen: Recombinant ovine IL-8 View All» Clone: 8M6 View All» Isotype: IgG2a View All» Species Reactivity:

• Bovine

• Goat

• Sheep
  View All» Application Notes: Capture antibody in ELISA. Recommended coating concentration 5 μg/mL. See suggested protocol. Flow cytometry: use 1 μg/test Neutralizes recombinant ovine IL-8. Western blotting Optimal working dilutions must be determined by the end user. Suggested ELISA Protocol For Ovine IL-8 1. Coat high capacity microtitre wells (e.g. Nunc maxisorp) with monoclonal anti-IL-8 antibody (Chemicon Catalog Number MAB1044) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4ºC. 2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST). 3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST. 4. Dilute rabbit anti-IL-8 (Chemicon Catalog Number AB1840) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST. 5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST. 6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulfuric acid. Notes: The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates. Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents. Assay sensitivity may be altered by adjusting incubation times and reagent concentrations. View All» Presentation: Liquid in PBS containing 0.1% sodium azide. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P10145 View All» Entrez Gene Number: NM_000584.2 View All» Gene Symbol:
  • IL8

  • GCP-1

  • emoctakin

  • MDNCF

  • NAP1

  • CXCL8

  • AMCF-I

  • IL-8

  • Emoctakin

  • TSG-1

  • NAP-1

  • LYNAP

  • MONAP

  • SCYB8

  • GCP1

  • LUCT

  • LECT

  • K60

  • 3-10C

  • b-ENAP

  • NAF
    View All» Alternate Names: IL-8 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • ELISA

    • Flow Cytometry

    • Neutralizing

    • Western Blotting
      View All» Entrez Gene Summary: The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. View All» UniProt Summary: FUNCTION: SwissProt: P10145 # IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.SIZE: 99 amino acids; 11098 Da SUBUNIT: Homodimer.SUBCELLULAR LOCATION: Secreted.PTM: Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.SIMILARITY: SwissProt: P10145 ## Belongs to the intercrine alpha (chemokine CxC) family. View All» Brand Family: Chemicon View All» Product Name: Anti-Interleukin-8 Antibody, clone 8M6 | MAB1044 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 250 µg View All» Format: Purified View All» Host: Mouse View All»
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