货号：MAB1059

品牌：Merck Millipore

规格：100Ug

目录价：￥5207.00

市场价格：￥4425.95

会员价格：￥4165.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2 | MAB1059 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes Pigment-Epithelium Derived Factor (PEDF). View All» Molecular Weight: ~46 kDa View All» Immunogen: Recombinant human GST-PEDF fusion protein. The sequence corresponding to aminoacids 196-347 of human PEDF tagged with GST on the N-terminal was expressed in E. Coli. GST-PEDF is about 46 kDa on SDS-PAGE. View All» Clone: 10F12.2 View All» Isotype: IgG2a View All» Background Information: Pigment epithelium-derived factor (PEDF) was first identified as a 50 kDa secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells (Tombran-Tink, 1991). More recently, PEDF was shown to have neurotrophic activity in the retina in vivo (Cayouette, 1999). PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells (Karakousis, 2001). The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. Recently, PEDF was shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo (Dawson, 1999; Stellmach, 2001). View All» Species Reactivity:

• Human

• Rat
  View All» Species Reactivity Note: Human. Expected to react with rat. View All» Application Notes: Western Blot: Recognizes a protein with a molecular weight of ~46 kDa when tested against immunogen. Western blot positive control is available (Catalog number AG672) ELISA: A previous lot of this antibody was used on ELISA.Immunocytochemistry: A previous lot of this antibody was used on Immunocytochemistry.Immunohistochemistry: A previous lot of this antibody was used on frozen sections.Optimal working dilutions must be determined by end user. View All» Control: Eye tissue. View All» Quality Assurance: Routinely evaluated by Western Blot on human placenta lysate.Western Blot Analysis: 1:1000 dilution of this lot detected pigment epithelium on 10 μg of human placenta lysate. View All» Purification Method: Protein A purfied View All» Presentation: Purified mouse monoclonal IgG2a in buffer containing 0.02 M PBS, pH 7.6, 0.25 M NaCl with 0.1% sodium azide. View All» Storage Conditions: Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt. View All» UniProt Number: P36955 View All» Entrez Gene Number: NM_002615.4 View All» Gene Symbol:
  • SERPINF1

  • Serpin-F1

  • EPC-1

  • PEDF

  • PIG35
    View All» Alternate Names:
    • pigment epithelium-derived factor

    • proliferation-inducing protein 35

    • serine (or cysteine) proteinase inhibitor, clade F (alpha-2antiplasmin, pigment epithelium derived factor), member 1

    • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin,pigment epithelium derived factor), member 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • ELISA

      • Immunocytochemistry

      • Immunohistochemistry

      • Western Blotting
        View All» UniProt Summary: FUNCTION: SwissProt: P36955 # Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.SIZE: 418 amino acids; 46342 Da SUBCELLULAR LOCATION: Secreted. Melanosome. Note=Enriched in stage I melanosomes.TISSUE SPECIFICITY: Retinal pigment epithelial cells and blood plasma.DEVELOPMENTAL STAGE: Expressed in quiescent cells.DOMAIN: SwissProt: P36955 The N-terminal (AA 44-121) exhibits neurite outgrowth- inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.PTM: The N-terminus is blocked.SIMILARITY: Belongs to the serpin family View All» Brand Family: Chemicon View All» Product Name: Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2 | MAB1059 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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