货号：MAB1137Z

品牌：Merck Millipore

规格：100Ug

目录价：￥5887.00

市场价格：￥5003.95

会员价格：￥4709.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Insulin Receptor Antibody, α subunit, azide free, clone 47-9 | MAB1137Z View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes a protein of 135kDa, identified as the alpha-subunit of insulin receptor (IR). Its epitope localizes in exon 7/8 and shows no cross-reaction with IGF-receptors {Soos, 1986}. Antibody superbly inhibits (>90%) insulin binding and BLOCKS insulin action. IR is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a beta-a-a-beta configuration. The beta-subunit (95kDa) possesses a single transmembrane domain, whereas the alpha-subunit is completely extracellular. The intracellular portion of the beta-subunit is a tyrosine-specific protein kinase with sequence homology to other such kinases. Activation of kinase is essential in signaling pathway whereby insulin regulates intracellular metabolism. View All» Epitope: alpha subunit, azide free View All» Immunogen: IM-9 lymphocytes followed by purified insulin receptor. View All» Clone: 47-9 View All» Isotype: IgG1 View All» Species Reactivity:

• Bovine

• Human

• Pig

• Sheep
  View All» Non-Reactive Species:
  • Mouse

  • Rat
    View All» Application Notes: Completely (>90%) Inhibits Insulin Binding: use antibody at 10nM Antagonist: Blocks insulin action Does not work in immunoprecipitation {Soos, 1986}. Optimal working dilutions must be determined by end user. View All» Applications Not Recommended:
    • Immunoprecipitation

    • Western Blotting
      View All» Control: 3T3 cells expressing transfected human insulin receptor View All» Purification Method: Protein G purfied View All» Presentation: Protein G Purified mouse immunoglobulin in 10mM PBS, pH 7.4 contain no preservatives. View All» Storage Conditions: Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» UniProt Number: P06213 View All» Entrez Gene Number:
      • NM_000208.2

      • NM_001079817.1
        View All» Gene Symbol:
        • INSR

        • CD220

        • HHF5

        • IR

        • EC 2.7.10.1 [Contains: Insulin receptor subunit alpha

        • Insulin receptor subunit beta].
          View All» Alternate Names:
          • CD220

          • MAB1137
            View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Affects Function View All» Entrez Gene Summary: After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. View All» UniProt Summary: FUNCTION: SwissProt: P06213 # This receptor binds insulin and has a tyrosine-protein kinase activity. Isoform Short has a higher affinity for insulin. Mediates the metabolic functions of insulin. Binding to insulin stimulates association of the receptor with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K). Can activate PI3K either directly by binding to the p85 regulatory subunit, or indirectly via IRS1.SIZE: 1382 amino acids; 156307 Da SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chains carry the kinase domain. Interacts with SORBS1 but dissociates from it following insulin stimulation. Binds SH2B2. Interacts with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. The sequences surrounding the phosphorylated NPXY motif contribute differentially to either IRS1 or SHC1 recognition. Interacts with the SH2 domains of the 85 kDa regulatory subunit of PI3K (PIK3R1) in vitro, when autophosphorylated on tyrosine residues. Interacts with SOCS7.SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.TISSUE SPECIFICITY: Isoform Long and isoform Short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin. Isoform Short is expressed also in the spleen and lymphoblasts.PTM: After being transported from the endoplasmic reticulum to the Golgi apparatus, the single glycosylated precursor is further glycosylated and then cleaved, followed by its transport to the plasma membrane. & Autophosphorylated on tyrosine residues in response to insulin. & Phosphorylation of Tyr-999 is required for IRS1- and SHC1- binding.DISEASE: SwissProt: P06213 # Defects in INSR are the cause of insulin resistance (Ins resistance) [MIM:125853]. & Defects in INSR are the cause of Rabson-Mendenhall syndrome [MIM:262190]; also known as Mendenhall syndrome. It is a severe insulin resistance syndrome characterized by insulin- resistant diabetes mellitus with pineal hyperplasia and somatic abnormalities. Typical features include coarse, senile-appearing facies, dental and skin abnormalities, abdominal distension, and phallic enlargement. Inheritance is autosomal recessive. & Defects in INSR are the cause of leprechaunism [MIM:246200]; also known as Donohue syndrome. Leprechaunism represents the most severe form of insulin resistance syndrome, characterized by intrauterine and postnatal growth retardation and death in early infancy. Inheritance is autosomal recessive. & Defects in INSR may be associated with noninsulin- dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. & Defects in INSR are the cause of familial hyperinsulinemic hypoglycemia 5 (HHF5) [MIM:609968]. Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. & Defects in INSR are the cause of insulin-resistant diabetes mellitus with acanthosis nigricans type A (IRAN type A) [MIM:610549]. This syndrome is characterized by the association of severe insulin resistance (manifested by marked hyperinsulinemia and a failure to respond to exogenous insulin) with the skin lesion acanthosis nigricans and ovarian hyperandrogenism in adolescent female subjects. Women frequently present with hirsutism, acne, amenorrhea or oligomenorrhea, and virilization. This syndrome is different from the type B that has been demonstrated to be secondary to the presence of circulating autoantibodies against the insulin receptor.SIMILARITY: SwissProt: P06213 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 2 fibronectin type-III domains. & Contains 1 protein kinase domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Insulin Receptor Antibody, α subunit, azide free, clone 47-9 | MAB1137Z View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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