货号：MAB1286

品牌：Merck Millipore

规格：100UL

目录价：￥5027.00

市场价格：￥4272.95

会员价格：￥4021.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Nuclear Spliceosomes Antibody, clone 780-3 | MAB1286 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Stains nuclear spliceosomes. Using indirect immunofluorescence, produces a speckled nuclear staining patter. Immunoelectron microscopy has shown that the antibody recognizes a discrete area of the nucleus identified as interchromatin granules. Immunoblots 105 kD molecular weight protein from human cells. Immunoprecipitates spliceosome and low-levels of snRNPs 1,2,5 (using 0.5 M salt). View All» Clone: 780-3 View All» Isotype: IgM View All» Species Reactivity: Human View All» Application Notes: Indirect immunofluorescence: 1:20-1:30. Use 30-100 μl per well/slide. Optimal working dilutions must be determined by the end user. In addition to the lymphnoid cultures, normal human epithelial cells can be screened by indirect immunofluorescence microscopy for positive reactions with the hybridoma supernatants. Since the human epithelial cells grow as monolayer cultures, they are plated directly onto the printed microscope slides after trypsinization and allowed to attach and grow overnight at 37°C in complete medium. The following day, the slides are briefly rinsed in PBS to remove the medium and the cells are fixed as described above. In general, the slides are not allowed to air dry either during or after the fixation procedure in order to maintain the cellular integrity and antigenicity of intracellular molecules. For photographic analysis, viable cell preparations obtained from Ficol™-hypaque gradient separations are cytocentrifuged directly onto slides at 1250 rpm for 10 minutes. This procedure flattens the lymphoid cells and greatly improves the visibility of intranuclear and cytoplasmic antigens. Slides prepared in this manner are fixed in the same way directly after cytocentrifugation. In order to screen the hybridoma supernatants by indirect immunofluorescence, 30-100 μL of each supernatant (optimize for each individual assay) are pipetted on a well of the printed microscope slides using a different tip for each supernatant. After 60 minutes of incubation at 37°C in a humidified chamber, the slides are rinsed 3 times with PBS at room temperature, and again incubated for 30 minutes at 37°C with 20 μL of a 1:20 dilution of fluorescein-conjugated goat anti-mouse IgG antibody (Millipore AP124F). The slides are then prepared rinsed 3 times with PBS, counterstained with Evans Blue for 5 minutes at room temperature using a freshly prepared solution containing 50 μL of a 1% stock solution of Evans Blue in 80 mL of PBS, rinsed a final time in PBS, and coverslipped using a 1:1 solution of glycerol: PBS. The slides are then examined by epifluorescence microscopy. Since many of the monoclonal antibodies produced a rapidly diminishing fluorescent reaction, exposure times optimally are less than five seconds. View All» Presentation: Hybridoma supernatant, concentrated by ammonium sulfate. Buffer: PBS with 0.1% sodium azide. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Western Blotting

• Immunohistochemistry

• Immunofluorescence
  View All» Brand Family: Chemicon View All» Product Name: Anti-Nuclear Spliceosomes Antibody, clone 780-3 | MAB1286 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Purified View All» Host: Mouse View All»
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