货号：3297

品牌：Merck Millipore

规格：

目录价：￥3196.00

市场价格：￥2716.60

会员价格：￥2556.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: LIGHT DIAGNOSTICS™ SimulFluor® Para 1,2/3, ~50 tests View All» Trade Name:

• LIGHT DIAGNOSTICS

• Chemicon (Millipore)
  View All» Qty/Pk: 2 mL View All» Product Overview: Intended Use Light Diagnostics™ SimulFluor® Parainfluenza 1, 2/3 Immunofluorescence Assay is intended for the simultaneous detection of parainfluenza 1 and 2, and identification of parainfluenza 3 following amplification in cell culture. For In Vitro Diagnostic Use. Summary and Explanation Respiratory viruses are responsible for a significant proportion of illness in human populations. Some viruses (e.g. influenza) are seasonal while others (e.g. adenovirus) predominantly affect different age groups. In a given population, respiratory viruses may be responsible for a considerable amount of morbidity. The advent of appropriate anti-viral therapy against some respiratory viruses makes rapid screening to identify these organisms imperative, allowing early institution of therapy. The two most widely used anti-viral agents today are amantadine/rimantadine for influenza A and ribavirin for respiratory syncytial virus bronchiolitis. Both agents have varying modes of action although most involve early infection steps such as penetration or uncoating (12,19). At physiologically attainable concentrations, amantadine and rimantadine specifically inhibit replication of influenza A (4). Ribavirin has broad spectrum activity in vitro against a host of viruses such as respiratory syncytial virus, measles, influenza A and B, and parainfluenza (7,13,14). Parainfluenza viruses, combined with respiratory syncytial virus, represent the most significant upper respiratory pathogens in infants and young children (1,2,3,5). Four types of parainfluenza virus have been identified in children and adults. Types 1 and 2 are major causes of laryngotracheo-bronchitis (croup). The severity of illness is greatest in children ages 2 to 4 years (6). Parainfluenza type 3 infection can also lead to croup and is second only to respiratory syncytial virus as a cause of infant bronchiolitis and pneumonia (8,9,10,11,15). Illness from type 3 infection is most severe in infants less than 1 year old (6). In older children and adults, illness may be asymptomatic or mimic the common cold (16). Severe croup in early childhood or infancy may lead to bronchial hyperactivity in older children or adolescents after exercise. However, it remains undetermined whether bronchial hyperactivity was a pre-existing condition, which played a role in the pathogenesis of croup or whether it developed as a complication of severe croup illness (17,18). Parainfluenza type 4 has been associated only with mild upper respiratory illness in adults and children and is difficult to identify in cell culture (6). Parainfluenza viruses belong to the genus Paramyxovirus of the family Paramyxoviridae. They are enveloped viruses with a single-strand RNA genome of negative polarity and range in diameter from 150 to 200 nm (20). Parainfluenza viruses grow well in primary simian or human kidney cell lines and in LLC-¬MK2, a rhesus kidney heteroploid cell line (21). Trypsin is needed in the medium for the recovery of types 1 and 2, but not 3. Virus infection of tissue culture can be recognized by hemadsorption of guinea pig erythrocytes. Types 2 and 3 can be recognized by syncytium formation. The most sensitive method for identifying parainfluenza viruses is culture isolation and confirmation; it is the standard method for most laboratories. Light Diagnostics™ SimulFluor® Parainfluenza 1, 2/ 3 Immunofluorescence Assay utilizes monoclonal antibodies to simultaneously detect parainfluenza 1 and 2, and identify parainfluenza 3 following amplification in culture to provide clear, easy-to-interpret results. Test Principle Light Diagnostics™ SimulFluor® Parainfluenza 1, 2/ 3 Immunofluorescence Assay utilizes a single reagent for the one step simultaneous detection of parainfluenza 1 and 2, and identification of parainfluenza 3 in infected cell culture. The Light Diagnostics™ SimulFluor® Para 1, 2/ 3 Reagent contains two components. The primary component is directed against parainfluenza 1 and 2; the second component is specifically directed against parainfluenza 3. The monoclonal antibodies in the components will bind to the appropriate viral antigen on the specimen slide. Unbound antibody is washed from the slide with phosphate buffered saline (PBS). Illumination with ultraviolet light allows visualization of the antigen-antibody complexes by fluorescence microscopy. Parainfluenza 1 and 2 infected cells will exhibit an apple-green fluorescence; while, parainfluenza 3 infected cells will exhibit a yellow-gold fluorescence. The primary component stains the parainfluenza 1 and 2 infected cells a bright apple-green but does not differentiate between these viruses. The secondary component stains the parainfluenza 3 infected cells bright yellow-gold and allows for the differentiation of parainfluenza 3 from parainfluenza 1 and 2 in a single well. Uninfected cells stain dull red due to the presence of Evans blue counterstain in the reagent. View All» Key Applications: Immunofluorescence View All» Usage Statement:
  • For in vitro Diagnostic Use

  • CE Mark
    View All» Components:
    • SimulFluor® Para 1, 2/ 3 Reagent - (Catalog No. 5297).

    • Para 1, 2 ,3 Control Slide - (Catalog No. 5011).

    • Phosphate Buffered Saline - (Catalog No. 5087).

    • Tween 20 / Sodium Azide Solution (100X) - (Catalog No. 5037).

    • Mounting Fluid - (Catalog No. 5013).
      View All» Brand Family: Chemicon View All» Format: SimulFluor View All» Host: Mouse View All» Storage Conditions: When stored at 2° to 8°C, the Light Diagnostics™ SimulFluor® Para 1, 2/ 3 Reagent is stable up to the expiration date printed on the kit label. Do not freeze or expose to elevated temperatures. Discard any remaining reagent after the kit expiration date. Warnings and Precautions • Sodium azide (present in the conjugate, monoclonal antibodies, wash buffer, and mounting fluid) can react with lead or copper plumbing to form potentially explosive metal azides. When disposing of these materials, flush with large volumes of water to prevent azide build-up. • Pooling or diluting conjugates or monoclonal antibodies may cause erroneous results. • Do not allow slides to dry at any time during the staining procedure. • Handle all specimens and materials as if potentially infectious. Decontaminate with 0.05% sodium hypochlorite (a 1:100 dilution of household bleach) prior to disposal. • Do not expose reagents to bright light during storage or incubation. • Avoid contact with Evans blue (present in the Light Diagnostics™ SimulFluor® Para 1, 2/ 3 Reagent), as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water. • Acetone is extremely flammable and harmful if swallowed or inhaled. Keep away from heat, sparks, or flames. Avoid breathing vapor. Use adequate ventilation. • Do not mouth pipette reagents. • Do not substitute reagents from other manufacturers. • Alteration of protocol provided may cause erroneous results. • When staining multiple samples on a slide, avoid cross contamination between samples. • Mounting Fluid contains a fluorescence enhancer that may be destructive to mucous membranes. Avoid direct skin or mucous membrane contact. If contact occurs, flush with large volumes of water. View All» Detection Methods: Fluorescent View All» Product Name: LIGHT DIAGNOSTICS™ SimulFluor® Para 1,2/3, ~50 tests View All» Materials Required but Not Delivered: 1. Cell culture for isolation of respiratory viruses: Each laboratory must maintain viable stocks of cells at appropriate passage state that will efficiently allow replication of respiratory viruses from processed patient specimens. These cells must be checked periodically for ability to support growth of respiratory viruses. Appropriate cell lines can be obtained from the American Type Culture Collection (ATCC), 12301 Parklawn Drive, Rockville, MD 20852. 2. Viral transport medium (VTM), which is non-inhibitory to the respiratory viruses and the tissue culture cells used for viral isolation: Hank's balanced salt solution (HBSS) with antibiotics and a protein stabilizer is a suitable medium. Avoid use of animal sera (except precolostral fetal bovine serum (FBS)) as protein stabilizer to prevent interference from inherent antibody. 3. Tissue culture media such as RPMI or Eagle's Minimum Essential Medium (EMEM) with appropriate amount of precolostral FBS can be used. 4. Sterile tissue culture tubes, dram vials, or multi well plates 5. Acetone, reagent grade or better Note: Acetone is hygroscopic and should be kept in tightly stoppered bottles. Presence of moisture in the acetone may result in a hazy appearance on the substrate during fluorescence microscopy. 6. Acetone-cleaned glass slides with wells at least 6mm in diameter in a hydrophobic mask 7. Sterile pipettes 8. Humid chamber 9. Sodium hypochlorite solution (0.05%) 10. No. 1 coverslips 11. 37°C incubator with rheostat for temperature regulation 12. Sterile swabs 13. Forceps 14. Vials for collection and transportation of specimens 15. Fluorescence microscope with appropriate filter combination for fluorescein (excitation peak 490 nm, emission peak 520 nm) 16. Sterile glass beads (1 to 3 mm diameter) 17. Centrifuge 18. Vortex mixer or sonicator 19. Distilled or deionized water View All» Antibody Type: Monoclonal Antibody View All»
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