货号：MAB13431

品牌：Merck Millipore

规格：1EA

目录价：￥6768.70

市场价格：￥5753.40

会员价格：￥5414.96

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MMP-2 Antibody, pro and active form, clone A-Gel VC2 | MAB13431 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: MAB13431 recognizes proteins of 72kDa and ~66kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2; also known as 72kDa collagenase IV or gelatinase A). CHEMICON MAB-13431 shows no cross-reaction with pro and active forms of other MMPs. CELLULAR LOCALIZATION: Cytoplasmic. View All» Epitope: pro and active form View All» Immunogen: Human APMA-activated native 72kDa Gelatinase A. View All» Clone: A-Gel VC2 View All» Isotype: IgG1 View All» Background Information: MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer or the stromal cells. There is a cooperation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction. View All» Species Reactivity: Human View All» Application Notes: Immunohistochemistry (Frozen & fixed-paraffin embedded) 2-4 μg/mL 30 min at RT. Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min. ELISA (not suitable for coating, contains BSA) 1:200-1:2000 Not suitable for Western blotting Optimal working dilution should be determined by the end user. View All» Applications Not Recommended: Western Blotting View All» Control: POSTIVE CONTROL: Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas. View All» Presentation: 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide View All» Storage Conditions: Antibody is stable for 12 months when stored at 2-8ºC View All» UniProt Number: P08253 View All» Entrez Gene Number: NM_004530.2 View All» Gene Symbol:

• MMP2

• TBE-1

• CLG4A

• CLG4

• MMP-II

• MONA

• MMP-2

• EC 3.4.24.24
  View All» Alternate Names: Gelatinase A; 72 kDa Type IV Collagenase View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • ELISA

  • Immunohistochemistry

  • Immunohistochemistry (Paraffin)
    View All» Entrez Gene Summary: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. View All» UniProt Summary: FUNCTION: SwissProt: P08253 # In addition to gelatin and collagens, it cleaves KiSS1 at a Gly- -Leu bond.COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.SIZE: 660 amino acids; 73882 Da SUBUNIT: Ligand for integrin alpha-V/beta-3.TISSUE SPECIFICITY: Produced by normal skin fibroblasts.DOMAIN: SwissProt: P08253 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.PTM: The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT- MMP3).DISEASE: SwissProt: P08253 # Defects in MMP2 are the cause of multicentric osteolysis nodulosis and arthropathy (MONA) [MIM:605156]. Inherited osteolyses or 'vanishing bone' syndromes are rare disorders of unknown etiology characterized by destruction and resorption of affected bones. MONA is an autosomal recessive osteolysis with multicentric involvement characterized by carpal and tarsal resorption, crippling arthritic changes, marked osteoporosis, palmar and plantar subcutaneous nodules and distinctive facies. & Defects in MMP2 are the cause of Winchester syndrome [MIM:277950]. Winchester syndrome is an autosomal recessive osteolysis syndrome. Winchester syndrome is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. The clinical and molecular findings suggest that Winchester syndrome and MONA are allelic disorders that form a continuous clinical spectrum.SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains. View All» Brand Family: Chemicon View All» Product Name: Anti-MMP-2 Antibody, pro and active form, clone A-Gel VC2 | MAB13431 View All» Concentration: 200 μg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 500 µL View All» Format: Purified View All» Host: Mouse View All»
    © 金山科研平台是专业的授权总代理区域代理经销平台。
    © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
    © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
    © 本文地址：https://www.16ao.com/thread-129914.htm
    © Merck Millipore代理MAB13431 Anti-MMP-2 Antibody, pro and active form, clone A-Gel VC2，产品报价联系微信jinshanbio