货号：MAB13435

品牌：Merck Millipore

规格：

目录价：￥4563.00

市场价格：￥3878.55

会员价格：￥3650.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MMP-2 Antibody, clone 7F3.2 | MAB13435 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Binds to Gelatinase-A. No cross reactivity with the other MMP family members (MMP-1, MMP-2B, MMP-3, and MMP-9). View All» Immunogen: Synthetic peptide based on the human sequence. View All» Clone: 7F3.2 View All» Isotype: IgG1 View All» Species Reactivity:

• Chicken

• Human
  View All» Application Notes: Ideal for Western Blotting and EIA applications. When used against the reduced protein, it identifies both a 72 kDa and a 68 kDa species (the pro-form and active form of the enzyme, respectively). Other applications have not been evaluated. Gelatinase-A is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen . The low protein levels produced in cell culture (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, G.I. et al. J. Biol. Chem. 267, 4583-4591. 1992). This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media. Recommended antibody dilution for Western Blots is 1:1,000 when using colorimetric substrates such BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be required for non-human samples. Optimal working dilutions must be determined by the end user. View All» Presentation: Liquid at 1 mg/mL containing sodium azide as a preservative. View All» Storage Conditions: The undiluted antibody solution is stable for approximately 6 months when stored -20°C. View All» UniProt Number: P08253 View All» Entrez Gene Number: NM_004530.2 View All» Gene Symbol:
  • MMP2

  • TBE-1

  • CLG4A

  • CLG4

  • MMP-II

  • MONA

  • MMP-2

  • EC 3.4.24.24
    View All» Alternate Names: Gelatinase A; 72 kDa Type IV Collagenase View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • ELISA

    • Western Blotting
      View All» Entrez Gene Summary: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. View All» UniProt Summary: FUNCTION: SwissProt: P08253 # In addition to gelatin and collagens, it cleaves KiSS1 at a Gly- -Leu bond.COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.SIZE: 660 amino acids; 73882 Da SUBUNIT: Ligand for integrin alpha-V/beta-3.TISSUE SPECIFICITY: Produced by normal skin fibroblasts.DOMAIN: SwissProt: P08253 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.PTM: The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT- MMP3).DISEASE: SwissProt: P08253 # Defects in MMP2 are the cause of multicentric osteolysis nodulosis and arthropathy (MONA) [MIM:605156]. Inherited osteolyses or 'vanishing bone' syndromes are rare disorders of unknown etiology characterized by destruction and resorption of affected bones. MONA is an autosomal recessive osteolysis with multicentric involvement characterized by carpal and tarsal resorption, crippling arthritic changes, marked osteoporosis, palmar and plantar subcutaneous nodules and distinctive facies. & Defects in MMP2 are the cause of Winchester syndrome [MIM:277950]. Winchester syndrome is an autosomal recessive osteolysis syndrome. Winchester syndrome is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. The clinical and molecular findings suggest that Winchester syndrome and MONA are allelic disorders that form a continuous clinical spectrum.SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains. View All» Brand Family: Chemicon View All» Product Name: Anti-MMP-2 Antibody, clone 7F3.2 | MAB13435 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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