货号：MAB1685

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Fodrin Antibody, clone FOD009 | MAB1685 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reacts with the intracellular fodrin (240 kDa) in human, mouse, rabbit, rat and bovine cells/tissue. In non-erythroid cells, a spectrin-like protein has been identified as fodrin and found to contain two subunits (alpha subunit of M.W. 240 kDa and beta subunit of M.W. 235 kDa). Fodrin is believed to be involved in 1) stabilizing membrane structure; 2) maintaining cell shape; 3) linking the cytoskeleton to plasma membranes or intracellular vesicles. In particular, fodrin is known to mediate the association of actin filaments with the plasma membrane. View All» Immunogen: Purified bovine brain fodrin View All» Clone: FOD009 View All» Isotype: IgG2a View All» Species Reactivity:

• Bovine

• Canine

• Human

• Mouse

• Pig

• Rat

• Rabbit
  View All» Application Notes: Western blotting at 1:200. ELISA: 1:1000. Immunoprecipitation: 1:100. Use rabbit capture secondary for best results. Immunocytochemistry: light fixation with triton X-100 or acetone is recommended. 1:100. Flow cytometry: 1:500 with permeabilization. Immunohistochemistry: 1:100 RIA: 1:5000 Note: Catalog number MAB1622 is a more reactive and better antibody in most cases than MAB1685, particularly in non-human samples. Optimal working dilutions must be determined by end user. SUGGESTED WESTERN BLOT PROTOCOL FOR MAB1685: 1) Prepare protein samples by using appropriate lysis buffer e.g. RIPA (50 mM Tris HCl, 150 mM NaCl 5 mM EDTA, 1% Triton X-100, 100 mg/mL PMSF, 10 mg/mL leupeptin 20 mg/mL aprotinin). 2) Separate protein samples in SDS-PAGE 6% or 7.5%. 3) Blot onto the nitrocellulose membrane with TRANS-BLOT CELL (Bio-Rad) using constant current 100 mA overnight (Transfer buffer: 12.1 g Tris, 43.2 g glycine, 800 mL methanol to total volume of 4 liters). 4) Block membrane with 3% BSA in TBS (2.42 Tris, 8 g NaCl to total volume 1 liter, pH 7.6) 1-2 hours at room temperature. 5) Incubate membrane in diluted primary antibody solution (diluted in TTBS: 0.1% Tween-20 in TBS) one hour. 6) Wash membrane with TTBS: two quick rinses, 1 x 15 min, 3 x 5 min. 7) Incubate membrane with diluted HRP-conjugated secondary antibody solution (TTBS) for one hour. 8) Wash membrane with TTBS: 2 quick rinses, 1 x 15 min, 4 x 5 min. 9) Detect the signal with detection reagent following the instruction of manufacture. View All» Presentation: Liquid in 10 mM sodium phosphate, 150 mM NaCl, 0.01% NaN3 pH 7.5. View All» Storage Conditions: Maintain frozen at -20°C for up to 12 months from date of receipt in undiluted aliquots. Avoid repeated freeze/thaw cycles. View All» UniProt Number: Q13813 View All» Entrez Gene Number: NM_003127.2 View All» Gene Symbol:
  • SPTAN1

  • FLJ44613

  • SPTA2

  • (ALPHA)II-SPECTRIN
    View All» Alternate Names: Spectrin View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • ELISA

    • Flow Cytometry

    • Immunoprecipitation

    • Radioimmunoassay

    • Western Blotting

    • Immunocytochemistry

    • Immunohistochemistry
      View All» UniProt Summary: FUNCTION: SwissProt: Q13813 # Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.SIZE: 2472 amino acids; 284539 Da SUBUNIT: Like erythrocyte spectrin, the spectrin-like proteins are capable of forming dimers which can further associate to tetramers. Interacts with isoform 1 of ACP1.SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.PTM: Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro (By similarity).SIMILARITY: SwissProt: Q13813 ## Belongs to the spectrin family. & Contains 3 EF-hand domains. & Contains 1 SH3 domain. & Contains 23 spectrin repeats. View All» Brand Family: Chemicon View All» Product Name: Anti-Fodrin Antibody, clone FOD009 | MAB1685 View All» Concentration: 500 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 50 µg View All» Format: Purified View All» Host: Mouse View All»
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