货号：07-2051

品牌：Merck Millipore

规格：

目录价：￥4997.00

市场价格：￥4247.45

会员价格：￥3997.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PEMT (C-terminus) View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the C-terminal region of human PEMT in Jurkat cell lysates. The antibody easily detected PEMT in Jurkat and L6 lysates by Western blotting, but expression was weak or not detected in A431, HeLa, HEK293, PC12, or NIH/3T3 cell lysates using very dilute antibody (0.25 μg/mL, data not shown). Positive signals in immunocytochemistry using higher concentrations of antibody (2.5 μg/mL) detected PEMT in these cell lines, suggesting differential expression levels of this protein in various cell lines. View All» Molecular Weight: ~22 kDa View All» Epitope: C-terminus View All» Immunogen: The immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PEMT. View All» Background Information: PEMT (phosphatidylethanolamine N-methyltransferase) is an an important enzyme for the synthesis of membrane phospholipids. PEMT converts phosphatidylethanolamine to phosphatidyl-choline by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Inhibition of hepatic PEMT activity has been associated with Alzheimer’s disease and schizophrenia. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with Human. Other homologies: Mouse (86% sequence homology) and Rat (73% sequence homology). View All» Application Notes: ELISA: This antibody was reported by an outside laboratory to be suitable for ELISAImmunocytochemistry:HeLa, A431, and NIH/3T3 (previous page) cells incubated with anti-PEMT (C-terminus) stained positively on nuclei and endoplasmic reticulum (ER). View All» Control: L6 and Jurkat lysates (Western blotting), HeLa, A431 cells (Immunocytochemistry) View All» Quality Assurance: Western Blot Analysis: 1:1,000 dilution of this antibody detected PEMT on 10 µg of L6 lysate (below) and Jurkat lysate (not shown). View All» Purification Method: Protein A chromatography View All» Presentation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. View All» Storage Conditions: Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance View All» UniProt Number: Q9UBM1 View All» Entrez Gene Number: NP_009100.2 View All» Gene Symbol:

• PEAMT

• PEMPT

• PEMT2

• PNMT
  View All» Alternate Names: phosphatidylethanolamine N-methyltransferase View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Western Blotting

  • Immunocytochemistry

  • ELISA
    View All» Entrez Gene Summary: This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Alternate splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq] View All» UniProt Summary: FUNCTION: Catalyzes three sequential methylation of phosphatidylethanolamine (PE) by AdoMet, thus producing phosphatidylcholine (PC).CATALYTIC ACTIVITY: S-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine.ENZYME REGULATION: The first methylation is rate-limiting.SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane proteinPotential. Mitochondrion membrane; Multi-pass membrane proteinPotential. Note= Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria By similarity.INVOLVEMENT IN DISEASE: Deletion of PEMT may be related to liver cancer.SEQUENCE SIMILARITIES: Belongs to the PEMT/PEM2 methyltransferase family. View All» Product Name: Anti-PEMT (C-terminus) View All» Concentration: 0.25 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Rabbit View All»
    © 金山科研平台是专业的授权总代理区域代理经销平台。
    © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
    © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
    © 本文地址：https://www.16ao.com/thread-130350.htm
    © Merck Millipore代理07-2051 ANTI-PEMT Antibody (C-Terminus)，产品报价联系微信jinshanbio