货号：MAB3129-250UG

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-BimL Antibody, clone 5E.5 | MAB3129-250UG View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Product Overview: Purified rat monoclonal antibody IgG2b Kappa isotype raised against recombinant GST-mouse BimL protein. The antibody recognises a reactive epitope that lies in the region that is present only in BimLong (BimL), one of the three splice variants of Bim. This epitope is absent in BimShort (BimS) and BimExtra long (BimEL). These antibodies can be used for easy detection of BimL by standard techniques including Western blot*, immunoprecipitation, immunocytochemistry, immunohistochemistry and FACS. It is highly sensitive and can, in most cases, recognise endogenous levels of BimL. View All» Specificity: The antibody recognizes a reactive epitope that lies in the region that is present only in BimLong (BIML), one of the three splice variants of Bim. This epitope is absent in BimShort (BimS) and BimExtra Long (BimEL). View All» Clone: 5E.5 View All» Isotype: IgG2b View All» Species Reactivity:

• Human

• Mouse
  View All» Application Notes: The antibody is intended for the detection of the long splice variant of Bim in a range of techniques including: Western blot immunoprecipitation FACS immunocytochemistry immunohistochemistry. It should be noted that the levels of antibody quoted are intended to serve as a guide only. The optimal concentration should be determined empirically by the end user. PROTOCOLS: Western blot In Western blot analysis it is recommended that the antibody be used at a concentration of 1mg/mL which has been found to detect 1x106 BimL expressing cells or 50ng of BimL recombinant protein. For the detection of endogenous BimL expression from either tissue** or cell lines**, it is recommended that the antibody be used at a concentration of 1-2mg/mL. FACS For FACS analysis of BimL overexpressing cell lines, it is recommended that the antibody be used at a concentration of 1-2mg/mL. It is necessary to permeabilise the cell membrane prior to analysis. Immunoprecipitation For immunoprecipitation of endogenous BimL from cell lines** or tissue**, it is recommended that the antibody be used at a concentration of 2mg/mL. Immunofluorescence In immunofluorescence techniques, it is recommended that the antibody be used at a concentration of 1-100mg/mL. The concentration should be determined empirically for each application. *Cross reactivity to a 26kDa cytosolic protein from various kidney cell lines has been noticed in Western blots only. The identity of this protein is not known. ** Examples of cell lines tested for endogenous Bim expression are the T lymphoma cell lines B6.2.16.BW2 or KO52.DA20, B cell lymphoma line ABL5.8.1 and human breast carcinoma cell line MCF-7. It is necessary to use cell lysate from 1x107 cells. For detection of Bim expression in tissue samples, it is necessary to use 1-5mg of total protein from an appropriate tissue source. View All» Presentation: Liquid. Antibody at a concentration of 1mg/mL containing 0.05% Bronidox as a preservative. >95% pure as determined by SDS-PAGE. View All» Storage Conditions: BimL antibody is shipped at ambient temperature. Store at 2-8°C, for up to 6 months. View All» UniProt Number: O43521 View All» Entrez Gene Number:
  • NM_138622.2

  • NM_138623.2

  • NM_138624.2

  • NM_138626.2

  • NM_138627.2

  • NM_207003.1

  • NM_006538.3

  • NM_138621.3

  • NM_207002.2
    View All» Gene Symbol:
    • BCL2L11

    • BimL

    • BIM

    • BIM-beta7

    • BOD

    • BAM

    • BimEL

    • BIM-beta6

    • BIM-alpha6
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • ELISA

      • Flow Cytometry

      • Immunoprecipitation

      • Western Blotting

      • Immunohistochemistry
        View All» Entrez Gene Summary: The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. View All» UniProt Summary: FUNCTION: SwissProt: O43521 # Induces apoptosis. Isoform BimL is more potent than isoform BimEL.SIZE: 198 amino acids; 22171 Da SUBUNIT: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including Mcl-1, Bcl-2, Bcl-X(L), BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK (By similarity).SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes (By similarity).DOMAIN: SwissProt: O43521 The BH3 motif is required for Bcl-2 binding and cytotoxicity. SIMILARITY: View All» Brand Family: Chemicon View All» Product Name: Anti-BimL Antibody, clone 5E.5 | MAB3129-250UG View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 250 µg View All» Format: Purified View All» Host: Rat View All»
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