货号：MAB3222

品牌：Merck Millipore

规格：100Ug

目录价：￥5215.00

市场价格：￥4432.75

会员价格：￥4172.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Bromodeoxyuridine Antibody, clone IIB5 | MAB3222 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reacts specifically with bromodeoxyuridine. It also recognizes BrdU when incorporated into nuclear DNA. View All» Immunogen: A fusion of mouse myeloma cells with spleen cells from a mouse intraperitoneally immunized with BrdU conjugated to bovine serum albumin. View All» Clone: IIB5 View All» Isotype: IgG1 View All» Background Information: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. View All» Species Reactivity: All View All» Application Notes: Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissues with acid/proteinase treatments. See: http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols. Flow Cytometry: 1:100-1:200; acid methanol treatment required. Optimal working dilutions must be determined by the end user. In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t) View All» Control: After incorporation of BrdU, all DNA containing species View All» Purification Method: Protein A purfied View All» Presentation: Protein A Purified mouse immunoglobulin in PBS with 0.09% sodium azide. View All» Storage Conditions: Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» Alternate Names: BrdU View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Flow Cytometry

• Immunocytochemistry

• Immunohistochemistry (Paraffin)
  View All» Brand Family: Chemicon View All» Product Name: Anti-Bromodeoxyuridine Antibody, clone IIB5 | MAB3222 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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  © Merck Millipore代理MAB3222 Anti-BrdU Antibody, clone IIB5;抗BrdU抗体，克隆IIB5store at -20℃，产品报价联系微信jinshanbio