货号：MAB3242

品牌：Merck Millipore

规格：100Ug

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Smoothelin Antibody, clone R4A | MAB3242 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: MAB3242 reacts with smoothelin, a cytoskeletal protein exclusively found in smooth muscle cells. Reacts with the 59 kDa and 100 kDa protein corresponding to smoothelin by Western blot. View All» Immunogen: Cytoskeletal extract of chicken gizzard. View All» Clone: R4A View All» Isotype: IgG1 View All» Background Information: Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cell with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin or vimentin. Smoothelin colocalizes with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 50 kDa isoform specific for visceral SMC, and an isoform with a molecular weight of approximately 100 kDa in vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.MAB3242 is useful to discriminate fully differentiated, contractile SMCs from proliferative SMCs. The distribution of smoothelin-positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions. View All» Species Reactivity:

• Canine

• Chicken

• Human

• Hamster

• Monkey

• Pig

• Feline
  View All» Application Notes: Western blot Immunocytochemistry Immunohistochemistry on both frozen and paraffin embedded tissue sections. For paraffin sections, high temperature antigen retrieval in 10 mM citrate buffer (pH 6.0) is highly recommended (van der Loop, 1997). Optimal working dilutions must be determined by the end user. View All» Presentation: Liquid in buffer (PBS) with 0.1% sodium azide. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P53814 View All» Entrez Gene Number:
  • NM_006932.3

  • NM_134269.1

  • NM_134270.1
    View All» Gene Symbol:
    • SMTN

    • smoothelin

    • Smoothelin.

    • FLJ38597

    • SMSMO

    • FLJ35365
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in three transcript variants. View All» UniProt Summary: FUNCTION: SwissProt: P53814 # Structural protein (cytoskeletal).SIZE: 917 amino acids; 99516 Da SUBCELLULAR LOCATION: Cytoplasm. Note=Exhibits a filamentous organization.TISSUE SPECIFICITY: Smooth muscle; contractile or vascular (for the long form).SIMILARITY: SwissProt: P53814 ## Contains 1 CH (calponin-homology) domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Smoothelin Antibody, clone R4A | MAB3242 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://www.16ao.com/thread-130920.htm
        © Merck Millipore代理MAB3242 Anti-Smoothelin Antibody, clone R4A;store at -20℃，产品报价联系微信jinshanbio