货号：MAB3290

品牌：Merck Millipore

规格：

目录价：￥4294.00

市场价格：￥3649.90

会员价格：￥3435.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PARP Antibody, clone 7A10 | MAB3290 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: This antibody only recognizes full-length form (p116) of PARP-1.Epitope: Region before the cleavage site of PARP-1. View All» Immunogen: A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. View All» Clone: 7A10 View All» Isotype: IgG1 View All» Background Information: PARP is a highly conserved nuclear enzyme present in higher eukaryotes. The enzyme is a Zn2+ -dependent DNA binding protein that recognizes DNA strand breaks and is implicated in DNA repair and in the apoptosis response of cells. As a marker for apoptosis, it has been shown that PARP cleavage mediated by caspase-3 activity occurs at the onset of apoptosis, and inhibition of PARP cleavage attenuates apoptosis in vitro. View All» Species Reactivity:

• Human

• Hamster

• Mouse

• Rat
  View All» Application Notes: Western Blot (1:500-1:2000)Flow Cytometry (1:200-1:400)Optimal working dilutions must be determined by end user.For Western blotting, sonication and urea are necessary to break up PARP/DNA interactions. To do this, resuspend cells in 62.5 mM Tris-HCl, pH 6.8, 6 M urea, 10% glycerol, 2% SDS, 0.00125% bromophenol blue and 5% beta-mercaptoethanol, sonicate for 15 seconds on full power and incubate at 65°C for 15 minutes, and then follow standard Western blotting procedures. Untreated cells should show only full-length PARP, while treated cells will show both cleaved and full-length PARP. View All» Presentation: Liquid in 10 mM HEPES sodium (pH 7.5), 150 mM NaCl, 100 ug/mL BSA, 50% glycerol and 0.03 sodium azide. View All» Storage Conditions: Maintain frozen at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P09874 View All» Entrez Gene Number: NM_001618.3 View All» Gene Symbol:
  • PARP1

  • poly(ADP-ribosyl)transferase

  • PARP-1

  • PARP

  • NAD

  • pADPRT-1

  • ADPRT1

  • Poly

  • ADPRT

  • PPOL

  • EC 2.4.2.30
    View All» Alternate Names:
    • Poly [ADP-ribose] polymerase 1

    • PARP-1

    • NAD(+) ADP-ribosyltransferase 1

    • ADPRT 1

    • Poly[ADP-ribose] synthase 1

    • ARTD1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Flow Cytometry
        View All» Entrez Gene Summary: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. View All» UniProt Summary: FUNCTION: SwissProt: P09874 # Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.SIZE: 1014 amino acids; 113084 Da SUBUNIT: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with ZNF423 (By similarity).SUBCELLULAR LOCATION: Nucleus.PTM: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. & Poly-ADP-ribosylated by PARP2.SIMILARITY: SwissProt: P09874 ## Contains 1 BRCT domain. & Contains 1 PARP alpha-helical domain. & Contains 1 PARP catalytic domain. & Contains 2 PARP-type zinc fingers. MISCELLANEOUS: The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. View All» Brand Family: Chemicon View All» Product Name: Anti-PARP Antibody, clone 7A10 | MAB3290 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 50 µg View All» Format: Purified View All» Host: Mouse View All»
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