货号：MAB3299

品牌：Merck Millipore

规格：50Ug

目录价：￥5677.00

市场价格：￥4825.45

会员价格：￥4541.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-DNA Antibody, single stranded specific, clone F7-26 | MAB3299 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Specifically reactive with single-stranded DNA and is ideal for the detection of apoptotic cells in suspension or in tissue sections. The antibody does not recognize DNA in double-stranded conformations. MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types. Antibody reacts specifically with deoxycytidine and requires stretch of ssDNA of at least 25-30 bases in length for the binding. Importantly, in contrast with the TUNEL method, monoclonal antibodies to ssDNA are specific for apoptotic cell death and do not detect necrotic cells. View All» Epitope: single stranded specific View All» Immunogen: F7-26 was generated by the immunization of mice with calf thymus single-stranded DNA. View All» Clone: F7-26 View All» Isotype: IgM View All» Background Information: Apoptosis is a genetically and biochemically regulated mechanism of programmed cell death that plays an important role in a variety of human diseases including cancer, immune disorders, and neurologic, cardiovascular and infectious diseases. Decreased stability of apoptotic DNA toward thermal denaturation is induced by the proteolysis of DNA-bound proteins during the execution of apoptosis. This conclusion is supported by the binding of anti-ssDNA monoclonal antibodies to non-apoptotic cells treated with proteinase K before heating and by prevention of DNA denaturation and MAb reactivity in apoptotic nuclei reconstituted with histones (Frankfurt et al. 1996, 1997). Thus, staining of cell suspensions and tissue sections with such antibodies following a heat treatment, which induces DNA denaturation in situ only in apoptotic nuclei, is a specific and sensitive method for the detection of apoptotic cells. CHEMICON MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types. View All» Species Reactivity: All View All» Application Notes: 1. Immunoprecipitation 2. Immunohistochemistry: formalin fixed, paraffin embedded tissues & 4% PF frozen tissues (special detection protocol required. FACS analysis (special protocol required), View All» Control: Apoptotic cells View All» Purification Method: Ammonium sulfate precipitation View All» Presentation: Purified Immunoglobulin. Liquid in PBS supplemented with 5% fetal bovine Serum (FBS), containing no preservatives. View All» Storage Conditions: Maintain frozen at -80°C in undiluted aliquots for up to 12 months after date of purchase. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Flow Cytometry

• Immunocytochemistry

• Immunohistochemistry

• Immunohistochemistry (Paraffin)
  View All» Brand Family: Chemicon View All» Product Name: Anti-DNA Antibody, single stranded specific, clone F7-26 | MAB3299 View All» Concentration: 100 μg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 50 μg View All» Format: Purified View All» Host: Mouse View All»
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  © Merck Millipore代理MAB3299 Anti-DNA Antibody, single stranded specific, clone F7-26;抗-DNA抗体，单链特异性，克隆 F7-26store at -80℃，产品报价联系微信jinshanbio