货号:MAB3299
品牌:Merck Millipore
规格:50Ug
目录价:¥5677.00
市场价格:¥4825.45
会员价格:¥4541.60
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Description:
Anti-DNA Antibody, single stranded specific, clone F7-26 | MAB3299
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Promotional Text:
Special Shipping Offer on Antibodies100% Performance Guaranteed
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Trade Name:
Chemicon (Millipore)
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Specificity:
Specifically reactive with single-stranded DNA and is ideal for the detection of apoptotic cells in suspension or in tissue sections. The antibody does not recognize DNA in double-stranded conformations. MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types. Antibody reacts specifically with deoxycytidine and requires stretch of ssDNA of at least 25-30 bases in length for the binding. Importantly, in contrast with the TUNEL method, monoclonal antibodies to ssDNA are specific for apoptotic cell death and do not detect necrotic cells.
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Epitope:
single stranded specific
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Immunogen:
F7-26 was generated by the immunization of mice with calf thymus single-stranded DNA.
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Clone:
F7-26
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Isotype:
IgM
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Background Information:
Apoptosis is a genetically and biochemically regulated mechanism of programmed cell death that plays an important role in a variety of human diseases including cancer, immune disorders, and neurologic, cardiovascular and infectious diseases. Decreased stability of apoptotic DNA toward thermal denaturation is induced by the proteolysis of DNA-bound proteins during the execution of apoptosis. This conclusion is supported by the binding of anti-ssDNA monoclonal antibodies to non-apoptotic cells treated with proteinase K before heating and by prevention of DNA denaturation and MAb reactivity in apoptotic nuclei reconstituted with histones (Frankfurt et al. 1996, 1997). Thus, staining of cell suspensions and tissue sections with such antibodies following a heat treatment, which induces DNA denaturation in situ only in apoptotic nuclei, is a specific and sensitive method for the detection of apoptotic cells. CHEMICON MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types.
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Species Reactivity:
All
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Application Notes:
1. Immunoprecipitation 2. Immunohistochemistry: formalin fixed, paraffin embedded tissues & 4% PF frozen tissues (special detection protocol required. FACS analysis (special protocol required),
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Control:
Apoptotic cells
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Purification Method:
Ammonium sulfate precipitation
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Presentation:
Purified Immunoglobulin. Liquid in PBS supplemented with 5% fetal bovine Serum (FBS), containing no preservatives.
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Storage Conditions:
Maintain frozen at -80°C in undiluted aliquots for up to 12 months after date of purchase.
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
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Flow Cytometry
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Immunocytochemistry
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Immunohistochemistry
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Immunohistochemistry (Paraffin)
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Brand Family:
Chemicon
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Product Name:
Anti-DNA Antibody, single stranded specific, clone F7-26 | MAB3299
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Concentration:
100 μg/mL
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Antibody Type:
Monoclonal Antibody
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Qty/Pk:
50 μg
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Format:
Purified
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Host:
Mouse
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