货号：03-100

品牌：Merck Millipore

规格：

目录价：￥4926.00

市场价格：￥4187.10

会员价格：￥3940.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: RIPAb+™ hnRNP M1-M4 View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Product Overview: RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.The hnRNP M1-M4 proteins are pre-mRNA-binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins associate with pre-mRNAs in the nucleus and influence pre-mRNA processing and other aspects of mRNA metabolism and transport. View All» Specificity: This antibody recognizes hnRNP M1-M4. View All» Molecular Weight: 64-68kDa View All» Immunogen: T7 gene 10 fusion protein containing full length human RNP M4 View All» Isotype: IgG1 View All» Background Information: Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis. View All» Species Reactivity:

• Bovine

• Human

• Mouse

• Pig

• Rat

• Rabbit
  View All» Application Notes: Immunoprecipitation from RIP lysate:Representative lot data.RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using 5 µg of either a normal mouse IgG, (Cat. # CS200621), or 5 µg of Anti-hnRNP M1-M4 antibody (Cat. # CS207316). Ten percent of the precipitated proteins (lane 1: normal mouse IgG, lane 2: hnRNP M1-M4) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-hnRNP M1-M4 (Cat. # CS207316, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231).Arrow indicates hnRNP M1-M4. (Figure 2).Automated Microfluidics-based Electrophoretic RNA Separation and Analysis (MFE):Representative lot data. RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either 1. normal mouse IgG (Cat. # CS200621), or 2. Anti-hnRNP M1-M4 antibody (Cat. # CS207316) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).Successful immunoprecipitation of hnRNP M1-M4-associated RNA was verified by automated microfluidics-based electrophoretic RNA separation and analysis. Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details. Electropherograms were generated by plotting fluorescence intensities versus migration times (Figure 3A). The virtual gel view was created from this data (Figure 3B).Western Blot Analysis: Representative lot data.HeLa nuclear extract was resolved by electrophoresis, transferred to PVDF and probed with anti-hnRNP M1-M4 (2 µg/mL). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates hnRNP M1-M4. (Figure 4). View All» Control: Includes negative control normal mouse IgG antibody and control primers specific for the cDNA of human hnRNP M. View All» Quality Assurance: RNA Binding Protein Immunoprecipitation:Representative lot data.RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal mouse IgG or 5 µg of Anti-hnRNP M1-M4 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).Successful immunoprecipitation of hnRNP M1-M4-associated RNA was verified by qPCR using RIP Primers hnRNP M, (Figure 1). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details. View All» Presentation: Anti-hnRNP M1-M4 (Mouse Monoclonal), Part # CS207316. One vial containing 50 μg of protein G purified mouse IgG1 in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide and 30% glycerol. Store at -20°C. Normal Mouse IgG, Part # CS200621. One vial containing 125 µg of purified mouse IgG in 125 µL of storage buffer containing 0.1% sodium azide. Store at -20°C.RIP Primers, hnRNP M, Part # CS207306. One vial containing 75 μL of 5 μM of each primer specific for human hnRNP M mRNA. Store at -20°C.FOR: GAG GCC ATG CTC CTG GGREV: TTT AGC ATC TTC CAT GTG AAA TCG View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: O43390 View All» Entrez Gene Number: NM_005968 View All» Gene Symbol:
  • HNRPR

  • hnRNP-R

  • FLJ25714

  • HNRNP-R

  • HNRNPR
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • RNA Binding Protein Immunoprecipitation (RIP)

    • Western Blotting
      View All» Entrez Gene Summary: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs and also contains a nuclear localization motif. Multiple alternatively spliced transcript variants have been found for this gene. View All» UniProt Summary: FUNCTION: SwissProt: O43390 # Component of ribonucleosomes, which are complexes of at least 20 other different heterogenious nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus.SIZE: 633 amino acids; 70943 Da SUBUNIT: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRNPA1, HNRNPA2B1, HNRPA3, HNRNPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRNPR, HNRNPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.SUBCELLULAR LOCATION: Nucleus, nucleoplasm.SIMILARITY: SwissProt: O43390 ## Contains 3 RRM (RNA recognition motif) domains. View All» Product Name: RIPAb+™ hnRNP M1-M4 View All» Concentration: 0.7 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 10 assays View All» Host: Mouse View All» Packaging: 10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context). View All»
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