货号：MAB3309

品牌：Merck Millipore

规格：

目录价：￥4013.00

市场价格：￥3411.05

会员价格：￥3210.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MMP-9 Antibody, near C-terminus, clone 56-2A4 | MAB3309 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Antibody MAB3309 is directed against the carboxy terminal domain of human MMP-9, residues 626-644. The antibody specifically reacts with the hMMP-9 and cross-reacts with guinea pig, rat and rabbit MMP-9, but not with the human MMP-1, 2, 3, or 13. The antibody does not react with mouse or bovine MMP-9. The antibody recognizes precursor (92 kDa) and active (83 kDa) forms of human and guinea pig MMP-9. View All» Molecular Weight: 92 & 83 kDa View All» Epitope: Near C-terminus View All» Immunogen: A peptide corresponding to amino acids 626-644 of human MMP-9 (RSASEVDRMFPGV PLDTHD). View All» Clone: 56-2A4 View All» Isotype: IgG1κ View All» Background Information: All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases). MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes. View All» Species Reactivity:

• Guinea Pig

• Human

• Rabbit

• Rat
  View All» Species Reactivity Note: Reacts with Human, Guinea Pig, Rat and Rabbit. Does not react with Mouse, Bovine and Hamster. Reactivity with other species has not been determined. View All» Non-Reactive Species:
  • Bovine

  • Mouse

  • Hamster
    View All» Application Notes: Immunoblotting: 2-5 µg/mL. Recognizes a band corresponding to pro-MMP-9 at 92kDa and an intermediate form at 83 kDa when evaluated versus the conditioned medium of human fibrosarcoma (HT1080) cells. Immunohistochemistry: A previous lot was used on frozen or paraffin-embedded tissue sections, PLP Fixation preferred (Nomura, 1996). 1-5 µg/mL of a previous lot of this antibody will work on formalin fixed tissues (see protocols).Optimal working dilutions must be determined by end user. View All» Control: HL60 cell lysate, U937 cell lysate, HT1080 cell lysate, human spleen lysate View All» Quality Assurance: Evaluated by Western Blot on Human spleen lysates.Western Blotting: 1:500 dilution of this antibody detected MMP-9 on 10 µg of Human spleen lysates. View All» Purification Method: Protein A purfied View All» Presentation: Purified mouse monoclonal IgG1κ in 0.1 M Phosphate buffer, pH 7.0 containing 2% protease free bovine serum albumin. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. View All» UniProt Number: P14780 View All» Entrez Gene Number: NM_004994.2 View All» Gene Symbol:
    • MMP9

    • MMP-9

    • GELB

    • CLG4B

    • EC 3.4.24.35 [Contains: 67 kDa matrix metalloproteinase-9 82 kDa matrix metalloproteinase-9].
      View All» Alternate Names: Matrix Metalloproteinase-9; Gelatinase B; 92 kDa Type IV Collagenase View All» Sales Restrictions: This product is not available for sale in Japan. View All» Usage Statement:
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

      • Manufactured by Daiichi Fine Chemical Co., Ltd
        View All» Key Applications:
        • ELISA

        • Immunohistochemistry

        • Immunohistochemistry (Paraffin)

        • Western Blotting
          View All» Entrez Gene Summary: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. View All» UniProt Summary: FUNCTION: SwissProt: P14780 # May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond.COFACTOR: Binds 2 zinc ions per subunit. & Binds 3 calcium ions per subunit.SIZE: 707 amino acids; 78427 Da SUBUNIT: Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form.TISSUE SPECIFICITY: Produced by normal alveolar macrophages and granulocytes.DOMAIN: SwissProt: P14780 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.PTM: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.MISCELLANEOUS: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status. View All» Brand Family: Chemicon View All» Product Name: Anti-MMP-9 Antibody, near C-terminus, clone 56-2A4 | MAB3309 View All» Concentration: 2.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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