货号：03-190

品牌：Merck Millipore

规格：

目录价：￥6242.00

市场价格：￥5305.70

会员价格：￥4993.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: HDAC1 (Hist-tag) human recombinant View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 50 µg View All» Product Overview: HDAC1 (Hist-tag) human recombinant produced in insect cells View All» Background Information: HDAC1 promotes transcriptional repression by deacetylating lysine ε-amino groups in histone N-terminal tails, a function frequently carried out in association with multi-protein transcription repression complexes such as NuRD3, Sin34 and CoREST6. Ubiquitously expressed in human tissues, HDAC1-containing complexes (at least class I) appear to contribute the greater part of deacetylase activity in HeLa nuclear extracts. Aside from its interaction with co-repressors, HDAC1 activity may be regulated by post-translational modifications, such as phosphorylation and sumoylation, or binding to the inhibitor maspin, a tumor-suppressive serpin homolog. Although originally described as a "histone deacetylase," HDAC1 has been shown to catalyze the regulatory deacetylation of non-histone proteins, including p53. Overexpression of HDAC1 has been found in various types of cancer. HDAC inhibitors (HDACi) have shown considerable promise as anti-cancer agents. View All» Key Applications: Enzyme Assay View All» Application Notes: HDAC deacetylase assays View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Species: Human View All» Molecular Weight: ~55 kDa View All» Brand Family: Upstate View All» Presentation: In liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol View All» Storage Conditions: Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC1 into separate tubes before storing at -80°C. View All» Product Name: HDAC1 (Hist-tag) human recombinant View All» Physical Form: Frozen solution View All» Format: Purified View All» Specific Activity: >1U/µg. 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity View All» Purity: Purity >50% by SDS-PAGE View All»