货号：MAB339

品牌：Merck Millipore

规格：100Ug

目录价：￥7203.00

市场价格：￥6122.55

会员价格：￥5762.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-GABA A Receptor α1 chain Antibody, N-terminus, clone BD24 | MAB339 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reacts with the alpha 1-chain of the GABAA receptor in bovine and human but not rat View All» Epitope: N-terminus View All» Immunogen: Purified GABA/benzodiazepine receptor from bovine cortex. View All» Clone: BD24 View All» Isotype: IgG1 View All» Species Reactivity:

• Avian

• Bovine

• Human

• Pig
  View All» Non-Reactive Species:
  • Rat

  • Feline
    View All» Application Notes: Immunohistochemistry: 10-20 μg/mL * See protocol below. Western blot: 20 μg/mL Immunoprecipitation Optimal working dilutions must be determined by end user. APPLICATION NOTES FOR MAB339 IMMUNOHISTOCHEMISTRY 1) Fresh tissue (human) should be used (3-6 hours postmortem). The tissue should be immersion fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50 mm sections and store in cryoprotective solution at -15°C. 2) Sections should be incubated floating in suitable small vials. 3) Block endogenous peroxidase with 100 mmol/L Tris-HCl, 150 mmol/L NaCl, pH 7.4 (Buffer A) containing 3% H2O2 (v/v) and 10% methanol for 20 min at room temperature. 4) Wash sections in Buffer A. Incubate sections in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v). 5) Wash sections in Buffer A. Incubate sections with MAB339 (diluted 10-20 mg/mL in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v) for 12-36 hours at +4°C 6) Wash sections in Buffer A. 7) Detect with standard secondary antibody detection system (PAP, ABC, etc.). 8) Wash sections in Buffer A. 9) Mount sections on chrome alum-coated slides, dry, dehydrate, and apply coverslips. View All» Presentation: Liquid. Buffer = 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide. View All» Storage Conditions: Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P14867 View All» Entrez Gene Number: NM_000806.4 View All» Gene Symbol:
    • GABRA1

    • ECA4

    • GABA

    • EJM
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry

      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. View All» UniProt Summary: FUNCTION: SwissProt: P14867 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.SIZE: 456 amino acids; 51802 Da SUBUNIT: Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.DISEASE: SwissProt: P14867 # Defects in GABRA1 are a cause of juvenile myoclonic epilepsy (JME) [MIM:606904]. JME is a common epileptic syndrome characterized by afebrile seizures, onset in adolescence (rather than in childhood) and myoclonic jerks. & Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. ECA4 is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Absence seizures may either remit or persist into adulthood.SIMILARITY: SwissProt: P14867 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family. View All» Brand Family: Chemicon View All» Product Name: Anti-GABA A Receptor α1 chain Antibody, N-terminus, clone BD24 | MAB339 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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