货号：MAB3393

品牌：Merck Millipore

规格：

目录价：￥4118.00

市场价格：￥3500.30

会员价格：￥3294.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Collagen Type V Antibody, clone IE2-E4 | MAB3393 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Product Overview: Collagen Type V Antibody is a monoclonal IgG2A antibody which binds specifically to Type V Collagen. Collagen Type V Antibody displays a high affinity for human, ovine, bovine, porcine, canine and kangaroo Type V Collagens. It displays moderate affinity for rabbit Type V Collagen. There is no evidence for cross reactivity with Collagen Types I, III and VI or connective tissue proteins (Elastin, Fibronectin and Laminin) at suggested working concentrations. View All» Clone: IE2-E4 View All» Isotype: IgG2a View All» Species Reactivity:

• Bovine

• Human

• Pig

• Sheep

• Kangaroo
  View All» Application Notes: ELISA: detection of native type V collagen. Capture: 2-4 μg/ml; Detection:A dilution of 10μg/mL of Collagen Type V Antibody will detect 50ng of human Collagen Type I with no reactivity with other Collagens. Higher levels of antibody may be necessary to detect lower concentrations of Collagen. Note antibody will not pair with itself. Immunohistochemistry:unfixed frozen sections: Cut 6 μm thick sections from frozen samples using a freezing microtome. Air-dry throughly. Rehydrate with PBS. Blocking is usually not necessary for IF studies, if using DAB, pretreatment for peroxidase is suggested and blocking 2% BSA in PBS will reduce back ground. Dilute Collagen Type V Antibody in PBS buffer, add to the sample(s) and incubate for 60 minutes. (1:100-1:500). Wash sections for 10 minutes in PBS, twice. Add FITC conjugated anti-mouse antibody and incubate for 60 minutes. Wash for 10 minutes in PBS, twice. Mount sections in glycerol/water (9:1 v/v) containing 1 mM 1,4-phenylenediamine. (for FITC stability or use Chemicon catalog numbers 5096 or 5013. NOTE: Preliminary studies suggest that enhanced staining of certain tissues maybe obtained by pretreatment (previous to step 2) of sections with various enzymes (eg 0.1% pepsin in 0.1M HCl, 37°C for 5 minutes). PROTOCOLS: ELISA A dilution of 15μg/mL of Collagen Type V Antibody will detect 15ng of bovine Collagen Type V with no cross reactivity with other Collagen Types. Higher levels of antibody may be necessary to detect lower concentrations of Collagen. Immunohistochemistry1-3 1. Cut 6μm thick sections from frozen samples using a freezing microtome. 2. Dilute Collagen Type V Antibody in PBS buffer, add to the sample(s) and incubate for 60 minutes. 3. Wash sections for 10 minutes in PBS, twice. Add FITC conjugated anti-mouse antibody and incubate for 60 minutes. 4. Wash for 10 minutes in PBS, twice. 5. Mount sections in glycerol/water (9:1 v/v) containing 1 mM 1,4-phenylenediamine. NOTE: Some tissues may require unmasking of the epitope using dilute (10 mM) Acetic Acid4 or collegenase. View All» Presentation: The immunoglobulin fraction has been purified from ascites fluid by Protein A Chromatography and shown to be >95% pure by Coomassie PAGE. 100μg purified antibody at a concentration of 1mg/mL in 50 mM Tris Acetate, 150 mM NaCl, 0.1% Bronidox (pH 4.5). View All» Storage Conditions: Collagen Type V Antibody is shipped, in liquid form, at ambient temperature. This material is stable for at least 6 months when stored at 2-8°C. View All» UniProt Number: P20908 View All» Entrez Gene Number: NM_000093.3 View All» Gene Symbol: COL5A1 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • ELISA

  • Western Blotting

  • Immunohistochemistry
    View All» Entrez Gene Summary: Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. View All» UniProt Summary: FUNCTION: SwissProt: P20908 # Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.SIZE: 1838 amino acids; 183560 DaSUBUNIT: Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4. Ref.8SUBUNIT LOCATION: Secreted › extracellular space › extracellular matrixBy similarity. PTM: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Sulfated on 40% of tyrosines.DISEASE:SwissProt: P20908 # Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 2 (EDS2) [MIM:130010]; also known as Ehlers-Danlos syndrome mitis. View All» Brand Family: Chemicon View All» Product Name: Anti-Collagen Type V Antibody, clone IE2-E4 | MAB3393 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
    © 金山科研平台是专业的授权总代理区域代理经销平台。
    © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
    © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
    © 本文地址：https://www.16ao.com/thread-131035.htm
    © Merck Millipore代理MAB3393 Anti-Collagen Type V, cln IE2-E4, (mouse monoclonal)，产品报价联系微信jinshanbio