货号：MAB3400

品牌：Merck Millipore

规格：40Ug

目录价：￥5666.00

市场价格：￥4816.10

会员价格：￥4532.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Vimentin Antibody, clone V9 | MAB3400 View All» Replaces: MAB3410 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes a 57-60 kDa protein. Shows no cross reactivity with other closely related intermediate filament proteins including desmin and GFAP. In tissue sections, this reagent shows positive reaction on connective tissue, endothelial cells, vascular smooth muscle cells and astrocytes. In tissue culture, typical intermediate filament profiles are seen in fibroblasts and other cell types, which contain vimentin View All» Molecular Weight: 57-60 kDa View All» Immunogen: Purified vimentin from pig eye lens View All» Clone: V9 View All» Isotype: IgG1 View All» Background Information: Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. View All» Species Reactivity:

• Bovine

• Canine

• Chicken

• Feline

• Human

• Monkey

• Pig

• Rat
  View All» Non-Reactive Species: Mouse View All» Application Notes: Immunoblotting:A dilution of 1:1000 was used in a previous lot.Immunohistochemistry: 5-10 μg/mL was used on a previous lot; can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antivbody reactivity.Immunocytochemistry: 5-10 μg/mL of a previous lot was used in IC.Optimal working dilutions must be determined by end user. View All» Control: Breast carcinoma tissue, Colon, Prostate, Tonsil, Tonsilar lymphoma, Uterus, Ureter View All» Quality Assurance: Evaluated by Western Blot on HeLa lysates.Western Blot: 1:1000 dilution of this antibody detected VIMENTIN on 10 μg of HeLa lysates View All» Purification Method: Protein A purfied View All» Presentation: Purified mouse monoclonal IgG1 presented in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8ºC from date of receipt. View All» UniProt Number: P08670 View All» Entrez Gene Number: NM_003380.2 View All» Gene Symbol:
  • VIM

  • FLJ36605
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunocytochemistry

    • Immunohistochemistry

    • Immunohistochemistry (Paraffin)

    • Western Blotting
      View All» Entrez Gene Summary: Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM] View All» UniProt Summary: FUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.SIZE: 466 amino acids; 53652 Da SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein.TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family. ... View All» Brand Family: Chemicon View All» Product Name: Anti-Vimentin Antibody, clone V9 | MAB3400 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 40 μg View All» Format: Purified View All» Host: Mouse View All»
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