货号：03-232

品牌：Merck Millipore

规格：1EA

目录价：￥7221.72

市场价格：￥6138.46

会员价格：￥5777.38

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Ubiquitin activating enzyme E1 (His-tag) human recombinant View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 50 µg View All» Product Overview: Human recombinant Ubiquitin activating enzyme E1 (His-tag) View All» Background Information: Ubiquitin activating enzyme E1 is responsible for the first step in the formation of the ubiquitin-protein isopeptide bond. It is a critical component for the initiation of in vitro conjugation reactions. E1 activates ubiquitin by first adenylating, with ATP, the C-terminal glycine carboxyl group of ubiquitin and thenlinking this residue to the sulphydryl side chain moiety of a cysteine residue in E1, by forming a high energy thiol ester bond and liberating free AMP. There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade. View All» Key Applications: Enzyme Assay View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Species: Human View All» Molecular Weight: ~119 kDa View All» Brand Family: Upstate View All» Source: Full length human ubiquitin-activating enzyme E1 containing a C-terminal His-tag View All» Presentation: In liquid containing 50 mM Tris-HCl, pH 7.5, containing 1.0 mM DTT View All» Storage Conditions: Stable for 12 months at -80°C from date of receipt. Avoid multiple freeze/thaw cycles. View All» Product Name: Ubiquitin activating enzyme E1 (His-tag) human recombinant View All» Physical Form: Frozen solution View All» Format: Purified View All» UniProt Number: P22314 View All» Host: E. coli View All»