货号：04-1007

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-FOXO3a Antibody, clone EP1949Y, Rabbit | 04-1007 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes FOXO3a at and around the N-terminus. View All» Molecular Weight: ~90 kDa View All» Epitope: N-terminus View All» Immunogen: Synthetic peptide corresponding to residues near the N-terminus of human FOXO3a. View All» Clone: EP1949Y View All» Isotype: IgG View All» Background Information: Forkhead box O3A (FOXO3A) is a member of the forkhead family of transcription factors (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. Upon oxidative stress, FOXO3A translocates to the nucleus where binding to the DNA sequence 5’-[AG]TAAA[TC]A-3’ occurs and IGF-1 is dephosphlorated, resulting in apoptosis. In order for the cell to survive, IGF-1 must be phosphlorated by AKT1/PKB at Thr32 and Ser-253, which will result in retention and inactivation of FOXO3A in the cytoplasm. Translocation of FOXO3A with the MLL gene has been associated with secondary acute leukemia. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, Mouse and Rat View All» Application Notes: Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected FOXO3a in human breast carcinoma tissue.Immunocytochemistry Analysis: A 1:50-100 dilution from a previous lot was used in IC. View All» Control: Jurkat cell lysate View All» Quality Assurance: Evaluated by Western Blot on Jurkat cell lysates.Western Blot Analysis: A 1:2,000-5,000 dilution of this antibody was used to detect FOXO3a in Jurkat cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing sodium azide, glycerol, and BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: O43524 View All» Entrez Gene Number: NP_001446 View All» Gene Symbol:
  • AF6q21

  • DKFZp781A0677

  • FKHRL1

  • FKHRL1P2

  • FOXO2

  • FOXO3A

  • MGC12739

  • MGC31925

  • OTTHUMP00000016944
    View All» Alternate Names:
    • AF6q21 protein

    • Forkhead in rhabdomyosarcoma-like 1

    • forkhead box O3

    • forkhead box O3A

    • forkhead homolog (rhabdomyosarcoma) like 1

    • forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. View All» UniProt Summary: FUNCTION: Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. SUBUNIT STURCTURE: Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation. SUBCELLULAR LOCATION: Cytoplasm › cytosol. Nucleus. Note: Translocates to the nucleus upon oxidative stress and in the absence of survival factors. TISSUE SPECIFICITY: Ubiquitous. PTM: In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. INVOLVEMENT IN DISEASE: A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.SEQUENCE SIMILARITIES: Contains 1 fork-head DNA-binding domain. View All» Product Name: Anti-FOXO3a, clone EP1949Y, Rabbit Monoclonal Antibody (04-1007) View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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