货号：MAB3502

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Angiotensin Converting Enzyme Antibody, clone 2E2 | MAB3502 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Denatured Angiotensin-converting enzyme [ACE]. View All» Immunogen: Angiotensin-converting enzyme (denatured) from human kidney View All» Clone: 2E2 View All» Isotype: IgG1 View All» Species Reactivity:

• Bovine

• Canine

• Guinea Pig

• Human

• Mouse

• Monkey

• Rat

• Rabbit

• Feline
  View All» Application Notes: Immunohistochemistry on paraffin embedded tissue: 5 μg/mL, with microwave antigen recovery. The method used for these antibodies is found in Metzer et al 1999, Kidney Int 56:1442-1454. Briefly, 2-4μm sections were cut followed by overnight drying in 37C. Dewaxed in xylene for 10min, followed by 10 in acetone and 10 min acetone/TBS 1:1. After this treatment the slides were washed in TBS, placed in microwave proofed tubes containing 0.01 citrate buffered solution pH7.0 and were boiled for 5 minutes 600W in the microwave. Evaporated volume was replaced with distilled water and the procedure repeated four times. After microwave treatment the slides were left to cool down and washed with TBS. (Control frozen sections were fixed in acetone 10 minutes and air-dried). The researchers used an ABC or APAAP enhancement to detect ACE. Other antibodies to ACE has been used successfully for: 1) the study of ACE expression during human pathology (myocardial infarction, pulmonary hypertension, atherosclerosis, kidney diseases Balyasnikova, Franke, Pauls, Metzer & Chenard-Neu references) as well as using animal models (Franke,Morrell 1997, Haberboshc references), 2) testicular cancer diagnostics (Falkenhahn, Morrel, 1995) and 3) differentiating mesothelioma from adenocarcinoma (Morrel, 1995). Western blotting: 5 μg/mL. Sensitivity: 100 mU/mL of human or rat ACE activity (1:20 dilution of human ACE (Catalog number AG 761) or rat ACE (Catalog number AG 782), respectively, which correspond to 100 ng/lane) Optimal working dilutions must be determined by the end user. View All» Presentation: Purified immunoglobulin. Liquid in PBS, pH 7.4, 150 mM NaCl. Contains no preservative. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P22966 View All» Entrez Gene Number:
  • NM_152831.1

  • NM_000789.2

  • NM_152830.1
    View All» Gene Symbol:
    • ACE

    • DCP

    • ACE1

    • carboxycathepsin

    • ACE-T

    • MGC26566

    • DCP1

    • CD143

    • EC 3.4.15.1

    • EC 3.2.1.- [Contains: Angiotensin-converting enzyme, testis- specific isoform, soluble form].

    • EC 3.4.15.1 [Contains: Angiotensin-converting enzyme, somatic isoform, soluble form].
      View All» Alternate Names: ACE; CD143; DCP1; Peptidyl-dipeptidase A; Peptidase P View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined. View All» UniProt Summary: FUNCTION: SwissProt: P22966 # Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.| P12821 # Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator.COFACTOR: Binds 1 zinc ion per subunit. & Binds 2 chloride ions per subunit.SIZE: 732 amino acids; 83330 Da SUBCELLULAR LOCATION: Angiotensin-converting enzyme, testis- specific isoform, soluble form: Secreted. & Cell membrane; Single-pass type I membrane protein.TISSUE SPECIFICITY: Spermatocytes, adult testis.DOMAIN: SwissProt: P22966 PTM: Phosphorylated by CK2 on Ser-725; which allows membrane retention (By similarity).DISEASE: SwissProt: P12821 # Genetic variations in ACE may be a cause of susceptibility to ischemic stroke [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. & Defects in ACE are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). & Genetic variations in ACE can influence susceptibility to diabetic nephropathy [MIM:603933]. Diabetic nephropathy is a kidney disease and resultant kidney function impairment due to the long standing effects of diabetes on the microvasculature (glomerulus) of the kidney. Features include increased urine protein and declining kidney function.SIMILARITY: SwissProt: P22966 ## Belongs to the peptidase M2 family.| P12821 ## Belongs to the peptidase M2 family.MISCELLANEOUS: The glycosidase activity probably uses different active site residues than the metalloprotease activity. & Inhibitors of ACE are commonly used to treat hypertension and cardiac dysfunction. View All» Brand Family: Chemicon View All» Product Name: Anti-Angiotensin Converting Enzyme Antibody, clone 2E2 | MAB3502 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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