货号：MAB3503

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Apaf-1 Antibody, clone 2E12 | MAB3503 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: The antibody recognizes the CARD (Caspase activation and recruitment domain) of human Apaf-1. No immunoreactivity to murine or monkey Apaf-1 has been observed. View All» Immunogen: Purified rat monoclonal antibody IgG2a Kappa isotype was raised against recombinant human Apaf-1 protein (AA 1-464). View All» Clone: 2E12 View All» Isotype: IgG2aκ View All» Species Reactivity: Human View All» Application Notes: FACS Analysis: For FACS analysis of Apaf-1 overexpressing cell lines, it is recommended that the antibody be used at a concentration of 0.5-1μg/ml. Immunoprecipitation: Recommend the antibody be used at a concentration of 1-20μg/ml. Immunohistochemistry: Recommend the antibody be used at 1μg/ml in overexpressing cell lines or 10μg/ml for detection of endogenous protein. A signal amplification strategy should be included when detecting endogenous levels of Apaf-1 (Hausmann et al., 2000). Western Blot: Recommend the antibody be used at a concentration of 1μg/mL, which has been found to detect endogenous Apaf-1 in 5x10E5 293T cells. ELISA: For detection of 1μg/ml of purified protein it is recommended that the antibody be used at a concentration of 0.5-1.0 μg/ml. View All» Presentation: Purified immunoglobulin by Protein A chromatography, is >95% pure by SDS-PAGE. The purified antibody is supplied in liquid form at a concentration of 1 mg/mL containing 0.05% Bronidox as a preservative. View All» Storage Conditions: Maintain undiluted aliquots at 2-8°C for up to 12 months after date of receipt. View All» UniProt Number: O14727 View All» Entrez Gene Number:

• NM_001160.2

• NM_013229.2

• NM_181861.1

• NM_181868.1

• NM_181869.1
  View All» Gene Symbol:
  • APAF1

  • CED4

  • DKFZp781B1145

  • Apaf-1

  • KIAA0413
    View All» Alternate Names: Apoptosis Protease-activating Factor 1 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • ELISA

    • Flow Cytometry

    • Immunoprecipitation

    • Western Blotting

    • Immunohistochemistry
      View All» Entrez Gene Summary: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. View All» UniProt Summary: FUNCTION: SwissProt: O14727 # Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.SIZE: 1248 amino acids; 141840 Da SUBUNIT: Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP.SUBCELLULAR LOCATION: Cytoplasm.TISSUE SPECIFICITY: Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.DOMAIN: SwissProt: O14727 The CARD domain mediates interaction with APIP.SIMILARITY: Contains 1 CARD domain. & Contains 1 NB-ARC domain. & Contains 13 WD repeats. View All» Brand Family: Chemicon View All» Product Name: Anti-Apaf-1 Antibody, clone 2E12 | MAB3503 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Rat View All»
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      © Merck Millipore代理MAB3503 Apaf-1 Monoclonal AB 100ug (Clone 2E12);已停产，产品报价联系微信jinshanbio