货号：04-1027

品牌：Merck Millipore

规格：

目录价：￥4037.00

市场价格：￥3431.45

会员价格：￥3229.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-SGK Antibody, clone Y238, Rabbit | 04-1027 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes SGK. View All» Molecular Weight: ~50-55 kDa View All» Immunogen: Synthetic peptide corresponding to residues of human SGK. View All» Clone: Y238 View All» Isotype: IgG View All» Background Information: Serum and glucocorticoid-regulated kinase (SGK-1) is a 50 kDa Serine/Threonine kinase that plays a role in the regulation of epithelial Na+ transport. Two additional SGK isoforms have been identified (SGK2 and SGK3). SGK is regulated by phosphorylation at Thr256 and Ser422 by the phosphatidyl-dependent protein kinase -1 (PDK1) and PDK-2 respectively. Nedd4-2 regulates SGK activity by catalyzing its ubiquination and degradation. SGK also regulates the binding of Nedd4-2 to the epithelial Na+ channel (ENaC) protein by phosphorylating Nedd4-2 which helps increase Na+ transport. While environmental stresses increases its expression, SGK is known to play a role in promoting cell survival, proliferation and differentiation. View All» Species Reactivity:

• Human

• Rat

• Mouse
  View All» Species Reactivity Note: Human, mouse, and rat. View All» Application Notes: Immunohistochemistry Analysis: A 1:50 dilution from a previous lot detected SGK in human kidney carcinoma tissue.Flow Cytometry: A 1:30 dilution from a previous lot was used in FC.Immunoprecipitation: A 1:50 dilution from a previous lot was used in IP. View All» Control: A431 and NIH/3T3 cell lysates View All» Quality Assurance: Evaluated by Western Blot on A431 and NIH/3T3 cell lysates.Western Blot Analysis: A 1:500 dilution of this antibody was used to detect SGK in A431 and NIH/3T3 cell lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: O00141 View All» Entrez Gene Number: NP_005618 View All» Gene Symbol:
  • EC 2.7.11.1

  • OTTHUMP00000017247

  • SGK
    View All» Alternate Names:
    • Serum/glucocorticoid-regulated kinase 1

    • serine/threonine protein kinase SGK

    • serum/glucocorticoid regulated kinase

    • serum/glucocorticoid regulated kinase 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry (Paraffin)

      • Immunoprecipitation

      • Flow Cytometry
        View All» Entrez Gene Summary: This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such ascell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. View All» UniProt Summary: FUNCTION: Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na+ transport than isoform 1. CATALTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.ENZYME REGULATION: Two specific sites, one in the kinase domain (Thr-256) and the other in the C-terminal regulatory region (Ser-422), need to be phosphorylated for its full activation.SUBUNIT STRUCTURE: Interacts with NEDD4 and NEDD4L. SUBCELLULAR LOCATION: Isoform 2: Cell membrane. Cytoplasm. Nucleus. Endoplasmic reticulum. Note: Nuclear, upon phosphorylation.TISSUE SPECIFICITY: Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle. INDUCTION: Isoform 1 is induced by serum and/or glucocorticoids. Isoform 2 is not induced by glucocorticoids. By excessive extracellular glucose and by TGF-beta, in cultured cells.DOMAIN: Isoform 2 subcellular localization at the plasma membane is mediated by the sequences within the first 120-amino acid.PTM: Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.Contains 1 AGC-kinase C-terminal domain.Contains 1 protein kinase domain. View All» Product Name: Anti-SGK, clone Y238, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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