货号：04-1036

品牌：Merck Millipore

规格：

目录价：￥3639.00

市场价格：￥3093.15

会员价格：￥2911.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Aurora-B (N-term) Antibody, clone EP1009Y, Rabbit | 04-1036 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Aurora-B at and around the N-terminus. View All» Molecular Weight: ~39 kDa View All» Epitope: N-terminus View All» Immunogen: Synthetic peptide corresponding to residues near the N-terminus of human Aurora-B. View All» Clone: EP1009Y View All» Isotype: IgG View All» Background Information: Aurora A, B, and C, are members of the serine/threonine kinase family. These have been identified as important regulators of the mitotic cell division process. Aurora B, specifically, is involved in the regulation of centrosome function, bipolar spindle assembly, and chromosome segregation. Overexpression of Aurora B has been linked to many functions in cancer development and may be potential effectors in the Ras pathway pertinent to oncogenesis. View All» Species Reactivity: Human View All» Species Reactivity Note: Human View All» Application Notes: Immunohistochemistry Analysis (paraffin): A 1:250-500 dilution from a previous lot detected Aurora-B in human hodgkins lymphoma carcinoma tissue. Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot worked in IC. Immunoprecipitation: A 1:50 dilution from a previous lot was used in IP. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot on HeLa cell lysates. Western Blot Analysis: A 1:5,000-50,000 dilution of this antibody was used to detect Aurora-B in HeLa cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q96GD4 View All» Entrez Gene Number: NP_004208.2 View All» Gene Symbol:

• AIK2

• AIM-1

• AIM1

• ARK2

• Aik2

• AurB

• Aurora-B

• EC 2.7.11.1

• IPL1

• STK-1

• STK12

• STK5

• aurora-1

• aurora-B
  View All» Alternate Names:
  • Aurora- and Ipl1-like midbody-associated protein 1

  • Aurora-related kinase 2

  • Aurora/IPL1-related kinase 2

  • aurora kinase B

  • serine/threonine kinase 12
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunohistochemistry (Paraffin)

    • Immunocytochemistry

    • Immunoprecipitation
      View All» Entrez Gene Summary: Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]) View All» UniProt Summary: FUNCTION: May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.COFACTOR: Magnesium.SUBUNIT STRUCTURE: Interacts with TACC1. Associates with RACGAP1 during M phase. Component of the CPC at least composed of BIRC5/survivin CDCA8/borealin, INCENP and AURKB/Aurora-B. Interacts with CDCA1 and NDC80. Interacts with EVI5. SUBCELLULAR LOCATION: Nucleus. Centromere. Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.TISSUE SPECIFICITY: High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. INVOLVEMENT IN DISEASE: Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. Contains 1 protein kinase domain. View All» Product Name: Anti-Aurora-B (N-term), clone EP1009Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Culture Supernatant View All» Host: Rabbit View All»
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