货号：04-1040

品牌：Merck Millipore

规格：

目录价：￥4095.00

市场价格：￥3480.75

会员价格：￥3276.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Actin Antibody, clone EP184E, Rabbit | 04-1040 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This actin antibody recognizes all muscle actin. View All» Molecular Weight: ~ 42 kDa View All» Epitope: N-terminus View All» Immunogen: Synthetic peptide corresponding to residues near the N-terminus of human Actin. View All» Clone: EP184E View All» Isotype: IgG View All» Background Information: Actin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kDa peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, mouse, and rat. View All» Application Notes: Immunoprecipitation: 1:20 dilution from a previous lot was used on IP.Flow Cytometry: 1:20 dilution from a previous lot was used on FC.Immunohistochemistry Analysis: 1:100 - 200 dilution from a previous lot detected Actin in human cardiac muscle tissue.Immunocytochemistry Analysis: 1:250 - 500 dilution from a previous lot detected Actin in HeLa cell lysate. View All» Control: A431 cell lysate View All» Quality Assurance: Evaluated by Western Blot on A431 cell lysate.Western Blot Analysis: 1:1000 dilution of this antibody was used to detect Actin in A431 cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P68032 View All» Entrez Gene Number: P68032 View All» Gene Symbol:
  • ACTC

  • CMD1R

  • CMH11
    View All» Alternate Names:
    • Alpha-cardiac actin

    • actin, alpha, cardiac muscle

    • actin, alpha, cardiac muscle 1

    • cardiac muscle alpha actin 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry (Paraffin)

      • Immunocytochemistry

      • Immunoprecipitation

      • Flow Cytometry
        View All» UniProt Summary: FUNCTION: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.SUBUNIT STRUCTURE: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. INVOLVEMENT IN DISEASE: Defects in ACTC1 are the cause of cardiomyopathy dilated type 1R (CMD1R) [MIM:102540]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in ACTC1 are the cause of cardiomyopathy familial hypertrophic type 11 (CMH11) [MIM:612098]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.SEQUENCE SIMILARITIES: Belongs to the actin family. View All» Product Name: Anti-Actin, clone EP184E, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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