货号：04-1046

品牌：Merck Millipore

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目录价：￥3615.00

市场价格：￥3072.75

会员价格：￥2892.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Notch1 (Cytoplasmic) Antibody, clone EP1238Y, Rabbit | 04-1046 View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Specificity: This antibody recognizes the cytoplasmic domain of Notch1 View All» Molecular Weight: ~125 kDa View All» Epitope: Cytoplasmic Domain View All» Immunogen: Synthetic peptide corresponding to the cytoplasmic domain of human Notch1 View All» Clone: EP1238Y View All» Isotype: IgG View All» Background Information: Notch is the receptor for Delta and Jagged ligands, which cause proteolysis of notch to liberate the intracellular domain. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved in numerous differentiation and cell lineage determination steps. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Human and mouse View All» Application Notes: Immunohistochemistry Analysis: 1:50 - 100 dilution from a previous lot detected Notch1 in Human fetal lung tissue. View All» Control: Hek293 cell lysates View All» Quality Assurance: Evaluated by Western Blotting on HEK293 cell lysates.Western Blotting Analysis: 1:500 dilution of this antibody detected Notch1 in HEK293 cell lysate. View All» Presentation: Rabbit monoclonal IgG in buffer containing 50mM Tris-Glycine (pH 7.4), 0.18 M NaCl containing 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P46531 View All» Entrez Gene Number: NP_060087.3 View All» Gene Symbol:
  • TAN1

  • hN1

  • notch1
    View All» Alternate Names:
    • Translocation-associated notch protein TAN-1

    • neurogenic locus notch homolog protein 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Immunohistochemistry
        View All» Entrez Gene Summary: This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq] View All» UniProt Summary: FUNCTION: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia By similarity.SUBUNIT STRUCTURE: Heterodimer of a C-terminal fragment N(TM) and an N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. Ref.6 Ref.7 Ref.8SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane proteinBy similarity. Notch 1 intracellular domain: NucleusBy similarity. Note= Following proteolytical processing NICD is translocated to the nucleus By similarity.TISSUE SPECIFICTY: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane By similarity.Phosphorylated By similarity.INVOLVEMENT IN DISEASE: NOTCH1 truncation is associated with T-cell acute lymphoblastic leukemia. Ref.13Defects in NOTCH1 are a cause of aortic valve disease [MIM:109730]. The disorder consists of an early developmental defect in the aortic valve and a later de-repression of calcium deposition that causes progressive aortic valve disease. Calcification of the aortic valve is the third leading cause of heart disease in adults. The incidence increases with age, and it is often associated with a bicuspid aortic valve present in 1-2% of the population. SEQUENCE SIMILARITIES: Belongs to the NOTCH family.Contains 5 ANK repeats.Contains 36 EGF-like domains.Contains 3 LNR (Lin/Notch) repeats. View All» Product Name: Anti-Notch1 (Cytoplasmic) Antibody, clone EP1238Y, Rabbit | 04-1046 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Host: Rabbit View All»
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