货号：MAB3608

品牌：Merck Millipore

规格：EA

目录价：￥3590.00

市场价格：￥3051.50

会员价格：￥2872.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Adiponectin Antibody, Globular Domain Region | MAB3608 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes mouse adiponectin, globular domain region. Recognizes natural and recombinant protein. SPECIES REACTIVITIES: Mouse. There is reactivity with rat (less than mouse) and weak reactivity with human adiponectin. Reactivity with other species has not been confirmed. View All» Epitope: Globular Domain Region View All» Immunogen: Recombinant mouse adiponectin conjugated to Fc expressed from mammalian cells. View All» Isotype: IgG1 View All» Background Information: Adipose tissue is highly specialized to play important roles in energy storage, fatty acid metabolism, and glucose homeostasis. Excess of adipose tissue in obesity and lack of adipose tissue in lipodystrophic states are associated with insulin resistance and type-2-diabetes in human and animal studies. Several groups have reported the identification of mouse and human transcript encoding an adipocyte-specific secretory protein originally termed Acrp30 (other names include AdipoQ, apM1, GBP28 and adiponectin). Adiponectin is a protein of 247 amino acids consisting of an N-terminal collagen-like domain and a C-terminal globular domain. The globular domain shares significant homology to the globular domains of type VIII and X collagens, subunits of the complement factor C1q as well as hib27, the latter a protein found in the serum of hibernating mice during non-hibernation. The crystal structures for the globular domain of adiponectin at 2.1 Å resolution reveals significant homology to TNF (tumor necrosis factor) family. Adiponectin circulates as a homotimer or larger complexes of 12-18 subunits. The concentration in serum is approximately 5-10 μg/mL, which corresponds to a maximum concentration of 10-30 nM for the higher order complex. Serum levels of adiponectin are significantly decreased in both obesity and type-2-diabetes, indicating a possible metabolic role or relationship to insulin resistance. In consistence with this, a recent report emphasized a proteolytic fragment (globular domain) of adiponectin to stimulate fatty acid oxidation by muscle. View All» Species Reactivity: Mouse View All» Application Notes: Western blot: 1:5,000-1:10,000 using ECL. Reacts with the ~30 kDa adiponectin protein. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature. Immunoprecipitation: 1-5 μg per sample in a final reaction volume of 500-1,000 μL. Suggested capture agent is Protein A. ELISA, direct and indirect: 1:5,000-1:10,000. Positive control is mouse plasma. Optimal working dilutions must be determined by the end user. View All» Presentation: Ascites fluid. Liquid View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. View All» UniProt Number: Q15848 View All» Entrez Gene Number: NM_004797.2 View All» Gene Symbol:

• ADIPOQ

• adiponectin

• AdipoQ

• apM-1

• APM1

• ACDC

• GBP28

• APM-1

• ACRP30

• apM1
  View All» Alternate Names: Acrp30; AdipoQ; apM1; GBP28 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. View All» UniProt Summary: FUNCTION: SwissProt: Q15848 # Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.SIZE: 244 amino acids; 26414 Da SUBUNIT: Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionnally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2.SUBCELLULAR LOCATION: Secreted.TISSUE SPECIFICITY: Synthesized exclusively by adipocytes and secreted into plasma.DOMAIN:SwissProt: Q15848 The C1q domain is commonly called the globular domain.PTM: Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. & HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin- sensitizing activity of adiponectin in hepatocytes (By similarity). & O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. & Not N-glycosylated.DISEASE: SwissProt: Q15848 # Defects in ADIPOQ are the cause of adiponectin deficiency [MIM:605441]. The result is a very low concentration of plasma adiponectin. Decreased adiponectin plasma levels are associated with obesity insulin resistance, and diabetes type 2.SIMILARITY: Contains 1 C1q domain. & Contains 1 collagen-like domain.MISCELLANEOUS: Variants Arg-84 and Ser-90 show impaired formation of HMW complexes whereas variants Cys-112 and Thr-164 show impaired secretion of adiponectin in any form. & HMW-complex blood contents are higher in females than in males, are increased in males by castration and decreased again upon subsequent testosterone treatment, which blocks HMW- complex secretion (By similarity). In type 2 diabetic patients, both the ratios of HMW to total adiponectin and the degree of adiponectin glycosylation are significantly decreased as compared with healthy controls. View All» Brand Family: Chemicon View All» Product Name: Anti-Adiponectin Antibody, Globular Domain Region | MAB3608 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Ascites View All» Host: Mouse View All»
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