货号：04-1061

品牌：Merck Millipore

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目录价：￥4680.00

市场价格：￥3978.00

会员价格：￥3744.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Neurofilament M (Ser614/Ser619) Antibody, clone EPR580(2)Y, Rabbit View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes phosphorylated Ser614/619 of Neurofilament M. View All» Molecular Weight: ~160 kDa View All» Epitope: Phosphorylated Ser614/Ser619 View All» Immunogen: Phospho-synthetic peptide corresponding to residues surrounding Serine 614/619 of human Neurofilament M. View All» Modifications: Phosphorylation View All» Clone: EPR580(2)Y View All» Isotype: IgG View All» Background Information: Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complex of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. View All» Species Reactivity: Human View All» Species Reactivity Note: Human View All» Control: Untreated and treated SHSY5Y cell lysate. View All» Quality Assurance: Evaluated by Western Blot on untreated and treated SHSY5Y cell lysates.Western Blot Analysis: 1:500 dilution of this antibody was used to detect Neurofilament M in untreated and treated with alkaline phosphatase SHSY5Y cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P07196 View All» Entrez Gene Number: NP_001099011.1 View All» Gene Symbol:

• CMT1F

• CMT2E

• FLJ53642

• NF-L

• NF68

• NFL

• neurofilament-light
  View All» Alternate Names:
  • 68 kDa neurofilament protein

  • Neurofilament triplet L protein

  • light molecular weight neurofilament protein

  • neurofilament protein, light chain

  • neurofilament subunit NF-L

  • neurofilament, light polypeptide

  • neurofilament, light polypeptide 68kDa
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunohistochemistry (Paraffin)
      View All» Entrez Gene Summary: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. View All» UniProt Summary: FUNCTION: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.SUBUNIT STRUCTURE: Interacts with RGNEF By similarity.DOMAIN: The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.PTM: O-glycosylated By similarity.INVOLVEMENT IN DISEASE: Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years). Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.MISCELLANEOUS: NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.SEQUENCE SIMILARITIES: Belongs to the intermediate filament family. View All» Product Name: Anti-phospho-Neurofilament M (Ser614/Ser619), clone EPR580(2)Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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