货号：04-1091

品牌：Merck Millipore

规格：

目录价：￥4902.00

市场价格：￥4166.70

会员价格：￥3921.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Sir2/SIRT1 Antibody, clone E104, Rabbit | 04-1091 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: The antibody does not cross-react with other sirtuin family members. View All» Molecular Weight: ~110 kDa View All» Epitope: C-terminus View All» Immunogen: Synthetic peptide corresponding to residues near C-terminus and before Ala-rich domain of human Sir2. View All» Clone: E104 View All» Isotype: IgG View All» Background Information: Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2α) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein. View All» Species Reactivity: Human View All» Species Reactivity Note: Human View All» Application Notes: Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Sir2 in human colon and lung squamous carcinoma tissue.Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP. View All» Control: Jurkat cell lysate View All» Quality Assurance: Evaluated by Western Blot on Jurkat cell lysates.Western Blot Analysis: A 1:5,000 dilution of this antibody was used to detect Sir2 in Jurkat cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q8IXJ6 View All» Entrez Gene Number: NP_001135970.1 View All» Gene Symbol:

• EC 3.5.1.-

• SIR2

• SIR2-like

• SIR2L

• SIR2L2
  View All» Alternate Names:
  • SIR2-like protein 2

  • silencing information regulator 2-like

  • silent information regulator 2

  • sir2-related protein type 2

  • sirtuin (silent mating type information regulation 2

  • homolog) 2 (S. cerevisiae)

  • sirtuin (silent mating type information regulation 2,

  • S.cerevisiae, homolog) 2

  • sirtuin 2

  • sirtuin type 2
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunoprecipitation

    • Western Blotting

    • Immunocytochemistry

    • Immunohistochemistry (Paraffin)
      View All» Entrez Gene Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. View All» UniProt Summary: FUNCTION: NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo. CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. COFACTOR: Binds 1 zinc ion per subunit.ENZYME REGULATION: Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide.SUBUNIT STRUCTURE: Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Note: Colocalizes with microtubules. TISSUE SPECIFICITY: Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. DEVELOPMENTAL STAGE: Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. PTM: Phosphorylated at the G2/M transition of the cell cycle. SEQUENCE SIMILARITIES: Belongs to the sirtuin family.Contains 1 deacetylase sirtuin-type domain.SEQUENCE CAUTION: The sequence AAF67015.1 differs from that shown. Reason: Frameshift at several positions. View All» Product Name: Anti-Sir2/SIRT1, clone E104, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://www.16ao.com/thread-131298.htm
      © Merck Millipore代理04-1091 Anti-Sir2/SIRT1, clone E104, Rabbit Monoclonal，产品报价联系微信jinshanbio