货号：MAB374

品牌：Merck Millipore

规格：200Ug

目录价：￥4899.00

市场价格：￥4164.15

会员价格：￥3919.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 | MAB374 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others. View All» Molecular Weight: 38 kDa View All» Immunogen: Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle. View All» Clone: 6C5 View All» Isotype: IgG1 View All» Background Information: Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). View All» Species Reactivity:

• Canine

• Human

• Mouse

• Rat

• Rabbit

• Fish

• Feline

• Pig
  View All» Species Reactivity Note: Human, porcine, canine, rat, mouse, rabbit, cat, and fish skeletal muscle. It has been reported that this antibody does not react with GAPDH from E. coli. View All» Non-Reactive Species: E. coli View All» Application Notes: ELISA: 1:1,000,000 dilution of a previous lot ws used for Immunoaffinity purification of GAPDH.Immunocytochemistry: 1:100 to 1:300 dilution of a previous lot was used in immunocytochemistry in PBS-BSA 10 mg/mL. 4% PFA, with 0.02% PBS-Triton X-100; 3 min RT (Griffoni, 2001).Western blot: 1:100 to 1:300. Recognizes a 36kDa band of the reduced monomer. Non-reduced GAPDH runs as a 146kDa tetramer. Immunoaffinity purification of GAPDH. Optimal working dilutions must be determined by end user. View All» Control: It is expressed in all cells. View All» Quality Assurance: Evaluated by Western Blot on A431 lysates.Western Blot Analysis: 1:500 dilution of this antibody detected GLYCERALDEHYDE-3-PDH on 10 μg of A431 lysates. View All» Purification Method: Protein A purfied View All» Presentation: Purified Mouse monoclonal IgG1 in PBS, pH 7.4 with 0.1% sodium azide. View All» Storage Conditions: Maintain at 2-8ºC in undiluted aliquots for up to 6 months after date of receipt. View All» UniProt Number: P04406 View All» Entrez Gene Number: NP_002037 View All» Gene Symbol: true View All» Alternate Names:
  • GAPDH

  • G3PDH

  • GAPD

  • OK/SW-cl.12

  • MGC88685

  • G3PD

  • EC 1.2.1.12
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • ELISA

    • Immunoprecipitation

    • Immunocytochemistry

    • Immunofluorescence

    • Immunohistochemistry

    • Western Blotting
      View All» Entrez Gene Summary: Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified. View All» UniProt Summary: FUNCTION: SwissProt: P04406 # Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.SIZE: 335 amino acids; 36053 Da SUBUNIT: Homotetramer. Binds PRKCI.SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.SIMILARITY: SwissProt: P04406 ## Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. View All» Brand Family: Chemicon View All» Product Name: Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 | MAB374 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 μg View All» Format: Purified View All» Host: Mouse View All»
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