货号：04-1102

品牌：Merck Millipore

规格：

目录价：￥3475.00

市场价格：￥2953.75

会员价格：￥2780.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-CD45 (C-term) Antibody, clone EP322Y, Rabbit | 04-1102 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes CD45 at and around the C-terminus. View All» Molecular Weight: ~180 kDa View All» Epitope: C-terminus View All» Immunogen: Synthetic peptide corresponding to residues in the C-terminal domain of human CD45. View All» Clone: EP322Y View All» Isotype: IgG View All» Background Information: CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. View All» Species Reactivity: Human View All» Species Reactivity Note: Human View All» Application Notes: Flow Cytometry Analysis: Flow cytometric analysis of permeabilized Jurkat cells using anti-CD45. View All» Control: Jurkat lysates View All» Quality Assurance: Evaluated by Western Blotting on Jurkat cell lysates.Western Blotting Analysis: 1:5,000 - 10,000 dilution of this antibody was used to detect CD45 in Jurkat cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P08575 View All» Entrez Gene Number: NP_002829.2 View All» Gene Symbol:

• B220

• CD45

• CD45R

• EC 3.1.3.48

• GP180

• L-CA

• LCA

• LY5

• T200

• glycoprotein
  View All» Alternate Names:
  • CD45 antigen

  • T200 glycoprotein

  • T200 leukocyte common antigen

  • leukocyte-common antigen

  • protein tyrosine phosphatase, receptor type, C

  • protein tyrosine phosphatase, receptor type, c polypeptide
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Flow Cytometry
      View All» Entrez Gene Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN. Ref.6 Ref.8Catalytic activity Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.SUBUNIT STRUCTURE: Binds GANAB and PRKCSH By similarity. Interacts with SKAP1.SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DOMAIN: The first PTPase domain interacts with SKAP1.PTM: Heavily N- and O-glycosylated.DISEASE: Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-B+NK+SCID) [MIM:608971]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Ref.14Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. Ref.13SEQUENCE SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.Contains 2 fibronectin type-III domains.Contains 2 tyrosine-protein phosphatase domains. View All» Product Name: Anti-CD45 (C-term) Antibody, clone EP322Y, Rabbit | 04-1102 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Unpurified View All» Host: Rabbit View All»
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