货号：04-1103

品牌：Merck Millipore

规格：

目录价：￥4118.00

市场价格：￥3500.30

会员价格：￥3294.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-E-cadherin Antibody, clone EP700Y, Rabbit | 04-1103 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes E-cadherin. View All» Molecular Weight: ~ 135/80 kDa View All» Immunogen: Synthetic peptide corresponding to residues in the 5th cadherin domain of human E-cadherin. View All» Clone: EP700Y View All» Isotype: IgG View All» Background Information: Cadherins are a family of transmembrane glycoproteins involved in Ca2+-dependent cell to cell interactions. The members of the family are differentially expressed in various tissues and function in the maintenance of tissue integrity, morphogenesis, and migration. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), placental cadherin (P-cadherin) and retinal cadherin (R-cadherin). Type II include kidney cadherin (K-cadherin) and osteoblast cadherin (OB-cadherin). One of the best characterized cadherins is E-cadherin (epithelial cadherin or cadherin-1). It consists of calcium binding extracellular domains responsible for interactions with other E-cadherin molecules on neighboring cells and interaction with integrin alphaEbeta7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia. View All» Species Reactivity: Human View All» Species Reactivity Note: Human View All» Application Notes: Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected E-cadherin in human breast carcinoma and hepatocellular carcinoma tissue.Immunocytochemistry: 1:500 dilution from a previous lot was used on IC.Immunoprecipitation: 1:50 dilution from a previous lot was used on IP. View All» Control: MCF-7 cell lysates. View All» Quality Assurance: Evaluated by Western Blot on MCF-7 cell lysates.Western Blot Analysis: 1:50,000 - 200,000 dilution of this antibody was used to detect E-cadherin in MCF-7 cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P12830 View All» Entrez Gene Number: P12830 View All» Gene Symbol:

• Arc-1

• CD324

• CDHE

• Cadherin-1

• E-cadherin

• ECAD

• LCAM

• UVO

• Uvomorulin

• uvomorulin
  View All» Alternate Names:
  • CAM 120/80

  • CD324 antigen

  • cadherin 1, E-cadherin (epithelial)

  • cadherin 1, type 1

  • cadherin 1, type 1, E-cadherin (epithelial)

  • calcium-dependent adhesion protein, epithelial

  • cell-CAM 120/80
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunohistochemistry (Paraffin)

    • Western Blotting

    • Immunocytochemistry

    • Immunoprecipitation
      View All» UniProt Summary: FUNCTION: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. SUBUNIT STRUCTURE: Homodimer; disulfide-linked. Interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs). Interacts with AJAP1, CTNND1 and DLGAP5. SUBCELLULAR LOCATION: Cell junction. Cell membrane; Single-pass type I membrane protein. Note: Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. TISSUE SPECIFICTIY: Non-neural epithelial tissues.PTM: During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disaaaembly of adherens junctions. INVOLVEMENT IN DISEASE: Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis.Defects in CDH1 are a cause of gastric cancer [MIM:137215]; also known as hereditary familial diffuse gastric cancer (HDGC).Defects in CDH1 are a cause of susceptibility to endometrial cancer [MIM:608089].Defects in CDH1 are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome.SEQUENCE SIMILARITIES: Contains 5 cadherin domains.SEQUENCE CAUTION: The sequence AAA61259.1 differs from that shown. Reason: Frameshift at positions 16, 22, 25, 28, 31, 34, 52, 67, 73, 76, 94, 102, 633 and 636. View All» Product Name: Anti-E-cadherin Antibody, clone EP700Y, Rabbit | 04-1103 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Target / Localization: Cell junctions View All» Format: Unpurified View All» Host: Rabbit View All»
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