货号：MAB3752

品牌：Merck Millipore

规格：

目录价：￥5148.00

市场价格：￥4375.80

会员价格：￥4118.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-LEF-1/TCF Antibody, HMG binding domain, clone REMB6 | MAB3752 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes Mobility Group DNA domain of LEF-1(Lymphoid Enhancer Factor-1). View All» Molecular Weight: 50/57 kDa View All» Epitope: HMG binding domain View All» Immunogen: The immunogen for the anti-LEF-1 antibody was a full length protein. View All» Clone: REMB6 View All» Isotype: IgG1 View All» Background Information: The immunogen for the anti-LEF-1 antibody was a full length protein. Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF- 4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kDa on SDSPAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms. Clone REMB1 recognizes the High Mobility Group DNA domain of LEF-1. View All» Species Reactivity:

• Human

• Mouse
  View All» Application Notes: Western blot (1-5 μg/ml) Immunofluoresnce Optimal working dilutions must be determined by end user. View All» Control: CCRF-HSB-2 (human leukemic lymphoblast) cell lysate, HuT 78 (cutaneous T lymphocyte) whole cell lysate, or Jurkat whole cell lysate View All» Presentation: Purified ascites. Provided as 0.2 μm sterile filtered solution in phosphate buffered saline with 0.08% sodiumazide. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. View All» UniProt Number: Q9UJU2 View All» Entrez Gene Number: NM_016269.2 View All» Gene Symbol:
  • LEF1

  • LEF-1

  • TCF1-alpha

  • TCF1ALPHA

  • DKFZp586H0919
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM] View All» Key Applications:
    • Immunofluorescence

    • Western Blotting
      View All» UniProt Summary: FUNCTION: SwissProt: Q9UJU2 # Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.SIZE: 399 amino acids; 44201 Da SUBUNIT: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds THOC4, MDFI and MDFIC.SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Found in nuclear bodies upon PIASG binding (By similarity).TISSUE SPECIFICITY: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines.DOMAIN:SwissProt: Q9UJU2 Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.SIMILARITY: Belongs to the TCF/LEF family. & Contains 1 HMG box DNA-binding domain. View All» Brand Family: Chemicon View All» Product Name: Anti-LEF-1/TCF Antibody, HMG binding domain, clone REMB6 | MAB3752 View All» Concentration: 1 mg/ml View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 250 µg View All» Format: Purified View All» Host: Mouse View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://www.16ao.com/thread-131328.htm
      © Merck Millipore代理MAB3752 Ms X Hu LEF-1/TCF，产品报价联系微信jinshanbio