货号：04-1123

品牌：Merck Millipore

规格：

目录价：￥4118.00

市场价格：￥3500.30

会员价格：￥3294.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-CD44 [H-CAM] Antibody, clone EPR1013Y, Rabbit | 04-1123 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes CD44. View All» Molecular Weight: ~ 85-90 kDa View All» Immunogen: Synthetic peptide corresponding to residues in human CD44. View All» Clone: EPR1013Y View All» Isotype: IgG View All» Background Information: Recent studies indicate that the expression of CD44 (H-CAM) on the cell surface changes profoundly during tumor metastasis, particularly during the progression of various carcinomas. Sequencing analysis reveals that CD44 exists in different forms (at least 15 isoforms have been identified) that are variants of the common or standard hemopoietic form CD44s. Specifically, the human CD44 gene contains 19 exons, out of which 10 exons can potentially be alternatively spliced. In particular, CD44s contains exons 1-15 and exons 15-19. The other exons (e.g. 6-14) are also spliced in a variety of different cells. It has been suggested that expression of CD44 variants with the additional exon 14 (so-called v10) is responsible for tumor progression and metastasis. In fact, CD44v10 expression has been used as an indicator of metastasis. View All» Species Reactivity: Human View All» Species Reactivity Note: Human View All» Application Notes: Immunohistochemistry Analysis: 1:100 - 250 dilution from a previous lot detected CD44 in human urinary bladder tissue.Immunocytochemistry: 1:100-250 dilution from a previous lot of this antibody was used in IC.Flow Cytometry: 1:30 dilution from a previous lot of this antibody was used in FC. View All» Control: TF-1 cell lysate. View All» Quality Assurance: Evaluated by Western Blot on TF-1 cell lysate.Western Blot Analysis: 1:5,000 dilution of this antibody was used to detect CD44 in TF-1 cell lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P16070 View All» Entrez Gene Number: P16070 View All» Gene Symbol:

• CD44R

• CDW44

• CDw44

• CSPG8

• ECMR-III

• Epican

• HCELL

• HUTCH-I

• IN

• LHR

• MC56

• MDU2

• MDU3

• MGC10468

• MIC4

• MUTCH-I

• PGP-1

• PGP-I

• Pgp1
  View All» Alternate Names:
  • CD44 antigen

  • CD44 antigen (homing function and Indian blood groupsystem)

  • CD44 molecule (Indian blood group)

  • CDW44 antigen

  • Extracellular matrix receptor III

  • GP90 lymphocyte homing/adhesion receptor

  • Heparan sulfate proteoglycan

  • Hermes antigen

  • Hyaluronate receptor

  • Phagocytic glycoprotein I

  • antigen gp90 homing receptor

  • cell adhesion molecule

  • cell surface glycoprotein CD44

  • chondroitin sulfate proteoglycan 8

  • extracellular matrix receptor-III

  • hematopoietic cell E- and L-selectin ligand

  • homing function and Indian blood group system
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunohistochemistry (Paraffin)

    • Western Blotting

    • Immunocytochemistry

    • Flow Cytometry
      View All» UniProt Summary: FUNCTION: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.SUBUNIT STRUCTURE: Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: An epithelial CD44E) is expressed by cells of epithelium and highly expressed by carcinomas. An hematopoietic CD44H) is expressed by cells of mesodermal origin. Expression is repressed in neuroblastoma cells.PTM: Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N-glycosylated. O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. POLYMORPHISM: CD44 is responsible for the Indian blood group system. The molecular basis of the In(A)=In1/In(B)=In2 blood group antigens is a single variation in position 46; In(B), the most frequent allele, has Arg-46.SEQUENCE SIMILARITIES: Contains 1 Link domain. View All» Product Name: Anti-CD44 [H-CAM], clone EPR1013Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://www.16ao.com/thread-131347.htm
      © Merck Millipore代理04-1123 Anti-CD44 [H-CAM], clone EPR1013Y, Rabbit Monoclonal，产品报价联系微信jinshanbio