货号：MAB377X

品牌：Merck Millipore

规格：500Ug

目录价：￥7049.00

市场价格：￥5991.65

会员价格：￥5639.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-NeuN Antibody, clone A60, Alexa Fluor®488 conjugated | MAB377X View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei). MAB377X reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. The immunohistochemical staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive with MAB377X include Purkinje, mitral and photoreceptor cells. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones. The antibody is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. View All» Immunogen: Purified cell nuclei from mouse brain. View All» Clone: A60 View All» Isotype: IgG1 View All» Background Information: NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992). View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Application Notes: Immunohistochemistry: 1:100 on rat (paraformaldehyde fixed) and mouse (paraformaldehyde fixed, antigen retrieved) brain tissue. Optimal working dilutions must be determined by end user. View All» Control: Brain tissue, most neuronal cell types throughout the adult nervous system View All» Purification Method: Protein A purfied View All» Presentation: Purified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide. View All» Storage Conditions: Maintain for 6 months at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» Gene Symbol:
  • NeuN

  • A60
    View All» Alternate Names: Neuron-Specific Nuclear Protein View All» Usage Statement:
    • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      View All» Key Applications: Immunohistochemistry View All» Brand Family: Chemicon View All» Product Name: Anti-NeuN Antibody, clone A60, Alexa Fluor®488 conjugated | MAB377X View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 500 μg View All» Format: AlexaFluor®488 View All» Host: Mouse View All»
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