货号：MAB380

品牌：Merck Millipore

规格：100UL

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Tubulin Antibody, β, clone 5H1 | MAB380 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Beta tubulin View All» Immunogen: Rat tubulin. View All» Clone: 5H1 View All» Isotype: IgM View All» Species Reactivity:

• Bovine

• Human

• Rat
  View All» Application Notes: Immunohistochemistry at 1:300-1:1,000 Optimal working dilutions must be determined by end user. IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB380 This antibody has been used successfully on 30 μm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows. 1) 3 x 10 minute washes in TBS (with or without 0.25% Triton). 2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody). 3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C. 4) 3 x 10 minute washes in TBS. 5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody). 6) 3 x 10 minute washes in TBS. 7) ABC Elite (1:200 Vector Labs) in TBS. 8) 2 x 10 minute washes in TBS. 9) 1 x 10 minute wash in phosphate buffer (no saline). 10) DAB reaction with 0.06% NiCl added for intensification. 11) 2 x 10 minute washes in PBS. 12) 1 x 10 minute wash in phosphate buffer (no saline). View All» Presentation: Ascites fluid containing sodium azide. Liquid View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: Q13509 View All» Entrez Gene Number: NM_006086.2 View All» Gene Symbol:
  • TUBB3

  • TUBB4

  • MC1R

  • beta-4
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunohistochemistry View All» UniProt Summary: FUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.SIZE: 450 amino acids; 50433 Da SUBUNIT: Dimer of alpha and beta chains.DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.SIMILARITY: Belongs to the tubulin family. View All» Brand Family: Chemicon View All» Product Name: Anti-Tubulin Antibody, β, clone 5H1 | MAB380 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Ascites View All» Host: Mouse View All»
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