货号：04-1139

品牌：Merck Millipore

规格：

目录价：￥3580.00

市场价格：￥3043.00

会员价格：￥2864.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-4E-BP1 (Thr70) Antibody, clone EPR654(2)AY, Rabbit | 04-1139 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody detects 4E-BP1 phosophorylated at Thr70. View All» Molecular Weight: ~17 kDa View All» Epitope: Phosphorylated Thr70 View All» Immunogen: Phospho-specific peptide corresponding to residues surrounding Thr70 of human 4E-BP1. View All» Modifications: Phosphorylation View All» Clone: EPR654(2)AY View All» Isotype: IgG View All» Background Information: 4E-BP1 is a eukaryotic translation initiation factor (eIF4E- binding protein), also known as PHAS-1. The binding of 4E-BP to eIF4E is regulated by phosphorylation and is reversible. Non-phosphorylated 4E-BP1 binds strongly to eIF4E; however, phosphorylated 4E-BP1 will not bind to eIF4E. Akt, TOR, MAP, S6, and Cdc2 kinases are able to inactivate 4E-BP1 by phosphorylation. 4E-BP1 undergoes phosphorylation which releases it from eIF4E, ultimately forming initiation complexes that aid in the upregulation of protein synthesis. View All» Species Reactivity: Human View All» Species Reactivity Note: Human View All» Control: 293T cell lysate View All» Quality Assurance: Evaluated by Western Blot on 293T cell lysates.Western Blot Analysis: A 1:500-1,000 dilution of this antibody was used to detect 4E-BP1 in 293T cell lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q13541 View All» Entrez Gene Number: NM_004095.3 View All» Gene Symbol:

• 4E-BP1

• 4EBP1

• BP-1

• MGC4316

• PHAS-I
  View All» Alternate Names:
  • Phosphorylated heat- and acid-stable protein regulated by insulin 1

  • eIF4E-binding protein 1

  • eukaryotic translation initiation factor 4E binding protein

  • 1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunohistochemistry (Paraffin)
      View All» Entrez Gene Summary: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. View All» UniProt Summary: FUNCTION: Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. SUBUNIT STUCTURE: Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts with RPTOR. PTM: Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR. SEQUENCE SIMILARITIES: Belongs to the eIF4E-binding protein family. View All» Product Name: Anti-phospho-4E-BP1 (Thr70), clone EPR654(2)AY, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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