货号：04-1141

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Chk2 (Ser33/Ser35) Antibody, clone EPR916(2)Y, Rabbit | 04-1141 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: The antibody only detects Chk2 phosphorylated on Ser33/35. View All» Molecular Weight: ~67 kDa View All» Epitope: Phosphorylated Ser33/Ser35 View All» Immunogen: Phospho-synthetic peptide corresponding to residues surrounding Ser33/35 of human Chk2. View All» Modifications: Phosphorylation View All» Clone: EPR916(2)Y View All» Isotype: IgG View All» Background Information: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer. View All» Species Reactivity: Human View All» Species Reactivity Note: Human View All» Application Notes: Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot was used in IC. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot on irradiated HeLa cell lysates.Western Blot Analysis: 1:500 dilution of this antibody was used to detect Chk2 in HeLa cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: O96017 View All» Entrez Gene Number: NM_007194 View All» Gene Symbol:

• CDS1

• CHK2

• Cds1

• EC 2.7.11.1

• HuCds1

• LFS2

• OTTHUMP00000028871

• PP1425

• RAD53

• bA444G7
  View All» Alternate Names:
  • CHK2 (checkpoint, S.pombe) homolog

  • CHK2 checkpoint homolog (S. pombe)

  • checkpoint-like protein CHK2

  • protein kinase CHK2

  • serine/threonine-protein kinase CHK2
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunocytochemistry
      View All» Entrez Gene Summary: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidlyphosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition,this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. View All» UniProt Summary: FUNCTION:Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'. CATALYTIC ACTIVITY:ATP + a protein = ADP + a phosphoprotein.COFACTOR: Magnesium.ENZYME REGULATION: Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation. Ref.13SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note: Isoform 10 is present throughout the cell. Isoform 4: NucleusIsoform 7: Nucleus Isoform 9: Nucleus Isoform 12: Nucleus TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.PTM: Phosphorylated by PLK4. INVOLVEMENT IN DISEASE: Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807].Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.Contains 1 FHA domain.Contains 1 protein kinase domain. View All» Product Name: Anti-phospho-Chk2 (Ser33/Ser35), clone EPR916(2)Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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