货号：04-1147

品牌：Merck Millipore

规格：

目录价：￥4118.00

市场价格：￥3500.30

会员价格：￥3294.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MCAM [CD146] Antibody, clone EPR3208, Rabbit | 04-1147 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes MCAM. View All» Molecular Weight: ~ 113 kDa View All» Immunogen: Synthetic peptide corresponding to residues in human MCAM. View All» Clone: EPR3208 View All» Isotype: IgG View All» Background Information: MCAM (MUC18 antigen, CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MCAM (Melanoma adhesion molecule) has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MCAM. The close structural relationship with N-CAM and related molecules suggests that MCAM may be also a developmentally regulated cell adhesion. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, mouse, and rat. View All» Application Notes: Immunohistochemistry Analysis: 1:250 - 500 dilution from a previous lot detected MCAM in melanoma tissue.Immunocytochemistry Analysis: 1:100-250 dilution from a previous lot was used on IC.Immunoprecipitation: 1:10 dilution from a previous lot was used on IP.Flow Cytometry: 1:80 dilution from a previous lot was used on FC. View All» Control: A375 cells, fetal artery, and HUVEC lysates. View All» Quality Assurance: Evaluated by Western Blot on A375 cell lysate, fetal artery, and HUVEC lysate.Western Blot Analysis: 1:10,000 dilution of this antibody was used to detect MCAM in A375 cell lysate, fetal artery, and HUVEC lysate. View All» Purification Method: Unpurified View All» Presentation: Cell culture supernatant containing Rabbit Monoclonal antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P43121 View All» Entrez Gene Number: P43121 View All» Gene Symbol:
  • CD146

  • Gicerin

  • MUC18
    View All» Alternate Names:
    • CD146 antigen

    • Cell surface glycoprotein P1H12

    • Melanoma-associated antigen A32

    • Melanoma-associated antigen MUC18

    • S-endo 1 endothelial-associated antigen

    • melanoma adhesion molecule

    • melanoma cell adhesion molecule
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Flow Cytometry

      • Immunoprecipitation

      • Immunohistochemistry (Paraffin)

      • Immunocytochemistry
        View All» UniProt Summary: FUNCTION: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.SEQUENCE SIMILARITIES: Contains 3 Ig-like C2-type (immunoglobulin-like) domains.Contains 2 Ig-like V-type (immunoglobulin-like) domains. View All» Product Name: Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Target / Localization: surface of pericytes from many different human tissues such as bone marrow, adipose, pancreas, and placenta View All» Format: Culture Supernatant View All» Host: Rabbit View All»
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