货号：04-1150

品牌：Merck Millipore

规格：

目录价：￥4118.00

市场价格：￥3500.30

会员价格：￥3294.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MMP-9 Antibody, clone EP1254, Rabbit | 04-1150 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes MMP-9. View All» Molecular Weight: ~ 92 kDa View All» Immunogen: Synthetic peptide corresponding to residues on human MMP-9. View All» Clone: EP1254 View All» Isotype: IgG View All» Background Information: All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn2+-binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion.MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes. The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas. View All» Species Reactivity:

• Human

• Rat
  View All» Species Reactivity Note: Human and rat. View All» Application Notes: Immunohistochemistry Analysis: 1:100 - 250 dilution from a previous lot detected MMP-9 in human gastric adenocarcinoma tissue.Immunocytochemistry Analysis: 1:250 - 500 dilution from a previous lot detected MMP-9 in HeLa cell lysate.Flow Cytometry: 1:1,000 dilution from a previous lot of this antibody was used in FC. View All» Control: U937, HL-60, and HT-1080 cell lysates View All» Quality Assurance: Evaluated by Western Blot on HeLa cell lysate.Western Blot Analysis: 1:5,000-20,000 dilution of this antibody was used to detect MMP-9 in U937, HL-60, and HT-1080 cell lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-glycine (pH 7.4), 0.15 M NaCl containing 40% glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P14780 View All» Entrez Gene Number: P14780 View All» Gene Symbol:
  • CLG4B

  • EC 3.4.24.35

  • GELB

  • MMP-9

  • OTTHUMP00000031674
    View All» Alternate Names:
    • 92 kDa gelatinase

    • 92 kDa type IV collagenase

    • 92kD type IV collagenase

    • Gelatinase B

    • macrophage gelatinase

    • matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

    • matrix metalloproteinase 9

    • matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase)

    • matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

    • type V collagenase
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry (Paraffin)

      • Western Blotting

      • Immunocytochemistry

      • Flow Cytometry
        View All» UniProt Summary: FUNCTION: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.CATALYTIC ACTIVITY: Cleavage of gelatin types I and V and collagen types IV and V.COFACTOR: Binds 2 zinc ions per subunit.Binds 3 calcium ions per subunit.ENZYME REGULATION: Inhibited by histatin-3 1/24 (histatin-5). SUBUNIT STRUCTURE: Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. SUBCELLULAR LOCATION: Secreted › extracellular space › extracellular matrix Probable. TISSUE SPECIFICITY: Produced by normal alveolar macrophages and granulocytes.INDUCTION: Activated by 4-aminophenylmercuric acetate and phorbol ester. DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.PTM: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.N- and O-glycosylated.INVOLVEMENT IN DISEASE: Defects in MMP9 may be a cause of susceptibility to lumbar disk herniation (LDH) [MIM:603932]. LDH is the predominant cause of low-back pain and unilateral leg pain. MISCELLANEOUS: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.SEQUENCE SIMILARITIES: Belongs to the peptidase M10A family.Contains 3 fibronectin type-II domains.Contains 4 hemopexin-like domains. View All» Product Name: Anti-MMP-9, clone EP1254, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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