货号：04-1151

品牌：Merck Millipore

规格：

目录价：￥3639.00

市场价格：￥3093.15

会员价格：￥2911.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Cyclin D1 Antibody, clone EPR2241(IHC)-32 Rabbit | 04-1151 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Cyclin D1. View All» Molecular Weight: ~36 kDa View All» Epitope: C-terminus View All» Immunogen: Synthetic peptide corresponding to residues near the C-terminus of human Cyclin D1. View All» Clone: EPR2241(IHC)-32 View All» Isotype: IgG View All» Background Information: Progression through G1 phase and transition from G1 to S phase of the cell division cycle is controlled by activation of a distinct series of serine/threonine kinase complexes which comprise of a cyclin regulatory subunit and a cyclin dependent kinase (cdk). D type cyclins are induced earlier than cyclin E in G1 and can form complexes with cdk2,-4,-5, and -6. Cyclin D1 is a nuclear protein during G1 phase and disappears from the nucleus in S-phase. Among the key cell cycle regulators, cyclin D1 has been implicated most strongly as a proto-oncogene in several human tumor types, including breast carcinomas. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, mouse, and rat. View All» Application Notes: Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Cyclin D1 in human mantle cell lymphoma tissue.Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.Immunoprecipitation Analysis: A 1:40 dilution from a previous lot was used in IP. View All» Control: MCF-7 cell lysate View All» Quality Assurance: Evaluated by Western Blot on MCF-7 cell lysates.Western Blot Analysis: A 1:10,000-50,000 dilution of this antibody was used to detect Cyclin D1 in MCF-7 cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P24385 View All» Entrez Gene Number: NP_444284.1 View All» Gene Symbol:
  • BCL1

  • D11S287E

  • PRAD1

  • U21B31
    View All» Alternate Names:
    • B-cell CLL/lymphoma 1

    • BCL-1 oncogene

    • G1/S-specific cyclin D1

    • PRAD1 oncogene

    • cyclin D1

    • cyclin D1 (PRAD1: parathyroid adenomatosis 1)
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Immunohistochemistry (Paraffin)

      • Immunoprecipitation
        View All» Entrez Gene Summary: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. View All» UniProt Summary: FUNCTION: Essential for the control of the cell cycle at the G1/S (start) transition.SUBUNIT STRUCTURE: Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. PTM: Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest.INVOLVEMENT IN DISEASE: A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas [MIM:168461]. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.A chromosomal aberration involving CCND1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with the IgH locus.SEQUENCE SIMILARITIES: Belongs to the cyclin family. Cyclin D subfamily. View All» Product Name: Anti-Cyclin D1, clone EPR2241(IHC)-32 Rabbit Monoclonal Antibody View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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